Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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Antimicrobial activity of jaborandi extract and sorbitol to oral microbes

Jaborandi extract와 Sorbitol이 구강미생물 증식에 미치는 영향

  • Jang, Jong-Hwa (Department of Dental Hygiene Science, a Graduate School, Hanseo University) ;
  • You, So-Yeon (Department of Dental Hygiene Science, a Graduate School, Hanseo University) ;
  • Oh, Tae-Jin (Department of Pharmaceutical Engineering, Sunmoon University)
  • 장종화 (한서대학교대학원 치위생학과) ;
  • 유소연 (한서대학교대학원 치위생학과) ;
  • 오태진 (선문대학교 제약공학과)
  • Received : 2013.05.22
  • Accepted : 2013.06.18
  • Published : 2013.06.30
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Abstract

Objectives : The aim of the study is to investigate the antimicrobial activity of jaborandi and sorbitol to various oral microbes. Methods : Jaborandi leaves contain pilocarpine. The conditions for extraction were optimized on the basis of substances, temperatures and pHs. Total pilocarpine content after extraction was analyzed by HPLC. The herbal antimicrobial activity of jaborandi and sorbitol were evaluated for oral microbes containing ATCC 25175 S. mutans, ATCC 13419 S. salivarius, ATCC 6249 S. mitis, ATCC 33398 S. equi, ATCC 29213 S. aureus, ATCC 14053 C. albicans. Results : The optimum conditions for highest yielding extraction were pilocarpine content after boiling at $100^{\circ}C$ for 1 hour at pH 3. The level of total pilocarpine content was analyzed at 833 mg/kg by HPLC. The most effective antimicrobial activity was obtained by combination of pilocarpine and sorbitol rather than pilocarpine, menthol and sorbitol, respectively. Conclusions : This results supported the preventive oral health care using safe and convenient jaborandi herb.

Keywords

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