Journal of Microbiology and Biotechnology

  • 제23권6호
  • /
  • Pages.856-863
  • /
  • 2013
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

IAA-Producing Penicillium sp. NICS01 Triggers Plant Growth and Suppresses Fusarium sp.-Induced Oxidative Stress in Sesame (Sesamum indicum L.)

  • Radhakrishnan, Ramalingam (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Shim, Kang-Bo (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Lee, Byeong-Won (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Hwang, Chung-Dong (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Pae, Suk-Bok (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Park, Chang-Hwan (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Kim, Sung-Up (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Lee, Choon-Ki (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Baek, In-Youl (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
  • 투고 : 2012.09.17
  • 심사 : 2013.02.24
  • 발행 : 2013.06.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

키워드

참고문헌

  1. Alabouvette, C., C. Olivain, Q. Migheli, and C. Steinberg. 2009. Microbiological control of soil-borne phytopathogenic fungi with special emphasis on wilt-inducing Fusarium oxysporum. New Phytol. 184: 529-544. https://doi.org/10.1111/j.1469-8137.2009.03014.x
  2. Alam, S. S., K. Sakamoto, and K. Inubushi. 2011. Biocontrol efficiency of Fusarium wilt diseases by a root-colonizing fungus Penicillium sp. Soil Sci. Plant Nutr. 57: 204-212. https://doi.org/10.1080/00380768.2011.564996
  3. Alwathnani, H. A., K. Perveen, R. Tahmaz, and S. Alhaqbani. 2012. Evaluation of biological control potential of locally isolated antagonist fungi against Fusarium oxysporum under in vitro and pot conditions. Afr. J. Microbiol. Res. 6: 312-319.
  4. Artursson, V., R. Finlay, and J. Jansson. 2006. Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth. Environ. Microbiol. 8: 1-10. https://doi.org/10.1111/j.1462-2920.2005.00942.x
  5. Bacon, C. W. and D. M. Hinton. 2007. Potential for control of seedling blight of wheat caused by Fusarium graminearum and related species using the bacterial endophyte Bacillus mojavensis. Biocontrol Sci. Technol. 17: 81-94. https://doi.org/10.1080/09583150600937006
  6. Barazani, O., M. Benderoth, K. Groten, C. Kuhlemeier, and I. T. Baldwin. 2005. Piriformospora indica and Sebacina vermifera increase growth performance at the expense of herbivore resistance in Nicotiana attenuata. Oecologia 146: 234-243. https://doi.org/10.1007/s00442-005-0193-2
  7. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  8. Chutima, R. and S. Lumyong. 2012. Production of indole-3- acetic acid by Thai native orchid-associated fungi. Symbiosis 56: 35-44. https://doi.org/10.1007/s13199-012-0158-2
  9. De-Vrije, T., N. Antoine, R. M. Buitelaar, S. Bruckner, M. Dissevelt, A. Durand, et al. 2001. The fungal biocontrol agent Coniothyrium minitans: Production by solid-state fermentation, application and marketing. Appl. Microbiol. Biotechnol. 56: 58-68. https://doi.org/10.1007/s002530100678
  10. El-Bramawy, M. A. S. 2006. Inheritance of resistance to Fusarium wilt in some crosses under field conditions. Plant Prot. Sci. 42: 99-105.
  11. Gajbhiye, A., A. R. Rai, S. U. Meshram, and A. B. Dongre. 2010. Isolation, evaluation and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum. World J. Microbiol. Biotechnol. 26: 1187-1194. https://doi.org/10.1007/s11274-009-0287-9
  12. Gill, S. S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48: 909-930. https://doi.org/10.1016/j.plaphy.2010.08.016
  13. Hariprasad, P., S. T. Divakara, and S. R. Niranjana. 2011. Isolation and characterization of chitinolytic rhizobacteria for the management of Fusarium wilt in tomato. Crop Prot. 30: 1606-1612. https://doi.org/10.1016/j.cropro.2011.02.032
  14. Hasan, H. A. H. 2002. Gibberellin and auxin-indole production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol. Immunol. Hung. 49: 105-118. https://doi.org/10.1556/AMicr.49.2002.1.11
  15. Heller, J. and P. Tudzynski. 2011. Reactive oxygen species in phytopathogenic fungi: Signaling, development, and disease. Annu. Rev. Phytopathol. 49: 369-390. https://doi.org/10.1146/annurev-phyto-072910-095355
  16. Heydari, A. and M. Pessarakli. 2010. A review on biological control of fungal plant pathogens using microbial antagonists. J. Biol. Sci. 10: 273-290. https://doi.org/10.3923/jbs.2010.273.290
  17. Hildebrandt, U., K. Janetta, and H. Bothe. 2002. Towards growth of arbuscular mycorrhizal fungi independent of a plant host. Appl. Environ. Microbiol. 68: 1919-1924. https://doi.org/10.1128/AEM.68.4.1919-1924.2002
  18. Jetiyanon, K. 2007. Defensive-related enzyme response in plants treated with a mixture of Bacillus strains (IN937a and IN937b) against different pathogens. Biol. Control 42: 178-185. https://doi.org/10.1016/j.biocontrol.2007.05.008
  19. Jyothi, B., N. A. Ansari, Y. Vijay, G. Anuradha, A. Sarkar, R. Sudhakar, and E. A. Siddiq. 2011. Assessment of resistance to Fusarium wilt disease in sesame (Sesamum indicum L.) germplasm. Aust. Plant Pathol. 40: 471-475. https://doi.org/10.1007/s13313-011-0070-x
  20. Kar, M. and D. Mishra. 1976. Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiol. 57: 315-319. https://doi.org/10.1104/pp.57.2.315
  21. Khamna, S., A. Yokota, and S. Lumyong. 2009. Actinomycetes isolated from medicinal plant rhizosphere soils: Diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World J. Microbiol. Biotechnol. 25: 649-655. https://doi.org/10.1007/s11274-008-9933-x
  22. Khan, A. L., M. Hamayun, N. Ahmad, J. Hussain, S. M. Kang, Y. H. Kim, et al. 2011. Salinity stress resistance offered by endophytic fungal interaction between Penicillium minioluteum LHL09 and Glycine max. L. J. Microbiol. Biotechnol. 21: 893-902. https://doi.org/10.4014/jmb.1103.03012
  23. Ksouri, R., W. Megdiche, A. Debez, H. Falleh, C. Grignon, and C. Abdelly. 2007. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiol. Biochem. 45: 244-249. https://doi.org/10.1016/j.plaphy.2007.02.001
  24. Kumar, S., P. Pandey, and D. K. Maheshwari. 2009. Reduction in dose of chemical fertilizers and growth enhancement of sesame (Sesamum indicum L.) with application of rhizospheric competent Pseudomonas aeruginosa LES4. Eur. J. Soil Biol. 45: 334-340. https://doi.org/10.1016/j.ejsobi.2009.04.002
  25. Kumazawa, S., T. Hamasaka, and T. Nakayama. 2004. Antioxidant activity of propolis of various geographic origins. Food Chem. 84: 329-339. https://doi.org/10.1016/S0308-8146(03)00216-4
  26. Latha, P., T. Anand, V. Prakasama, E. I. Jonathan, M. Paramathma, and R. Samiyappan. 2001. Combining Pseudomonas, Bacillus and Trichoderma strains with organic amendments and micronutrient to enhance suppression of collar and root rot disease in physic nut. Appl. Soil Ecol. 49: 215-223.
  27. Manikandan, R., D. Saravanakumar, L. Rajendran, T. Raguchander, and R. Samiyappan. 2010. Standardization of liquid formulation of Pseudomonas fluorescens Pf1 for its efficacy against Fusarium wilt of tomato. Biol. Control 54: 83-89. https://doi.org/10.1016/j.biocontrol.2010.04.004
  28. Miransari, M. 2011. Soil microbes and plant fertilization. Appl. Microbiol. Biotechnol. 92: 875-885. https://doi.org/10.1007/s00253-011-3521-y
  29. Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  30. Pavlo, A., O. Leonid, Z. Iryna, K. Natalia, and P. A. Maria. 2011. Endophytic bacteria enhancing growth and disease resistance of potato (Solanum tuberosum L.). Biol. Control 56: 43-49. https://doi.org/10.1016/j.biocontrol.2010.09.014
  31. Peskan-Berghoefer, T., B. Shahollari, P. H. Giong, S. Hehl, C. Markert, V. Blanke, et al. 2004. Association of Piriformospora indica with Arabidopsis thaliana roots represents a novel system to study beneficial plant-microbe interactions and involves early plant protein modifications in the endoplasmic reticulum and at the plasma membrane. Physiol. Plant. 122: 465-477. https://doi.org/10.1111/j.1399-3054.2004.00424.x
  32. Pimentel, D. and L. Levitan. 1986. Pesticides: Amounts applied and amounts reaching pests. Bioscience 36: 86-91. https://doi.org/10.2307/1310108
  33. Ruanpanun, P., N. Tangchitsomkid, K. D. Hyde, and S. Lumyong. 2010. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: Screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production. World J. Microbiol. Biotechnol. 26: 1569-1578. https://doi.org/10.1007/s11274-010-0332-8
  34. Shoresh, M., G. E. Harman, and F. Mastouri. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annu. Rev. Phytopathol. 48: 21-43. https://doi.org/10.1146/annurev-phyto-073009-114450
  35. Sundaramoorthy, S., T. Raguchander, N. Ragupathi, and R. Samiyappan. 2012. Combinatorial effect of endophytic and plant growth promoting rhizobacteria against wilt disease of Capsicum annum L. caused by Fusarium solani. Biol. Control 60: 59-67.
  36. Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731-2739. https://doi.org/10.1093/molbev/msr121
  37. Ting, A. S. Y., S. W. Mah, and C. S. Tee. 2012. Evaluating the feasibility of induced host resistance by endophytic isolate Penicillium citrinum BTFO8 as a control mechanism for Fusarium wilt in banana plantlets. Biol. Control 61: 155-159. https://doi.org/10.1016/j.biocontrol.2012.01.010
  38. Vujanovic, V., D. Mavragani, and C. Hamel. 2012. Fungal communities associated with durum wheat production systems: A characterization by growth stage, plant organ and preceding crop. Crop Prot. 37: 26-34. https://doi.org/10.1016/j.cropro.2012.02.006
  39. Wu, H. S., X. N. Yang, J. Q. Fan, W. G. Miao, N. Ling, Y. C. Xu, et al. 2009. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms. Biol. Control 54: 287-300.

피인용 문헌

  1. Characterization of plant growth-promoting traits of Penicillium species against the effects of high soil salinity and root disease vol.9, pp.1, 2013, https://doi.org/10.1080/17429145.2014.930524
  2. Indole-3-Acetic Acid-Producing Yeasts in the Phyllosphere of the Carnivorous Plant Drosera indica L vol.9, pp.12, 2013, https://doi.org/10.1371/journal.pone.0114196
  3. The Potency of Endophytic Fungal Isolates Collected from Local Aromatic Rice as Indole Acetic Acid (IAA) Producer vol.3, pp.None, 2015, https://doi.org/10.1016/j.profoo.2015.01.009
  4. Cucumber performance is improved by inoculation with plant growth-promoting microorganisms vol.65, pp.1, 2013, https://doi.org/10.1080/09064710.2014.960889
  5. Penicillium menonorum: A Novel Fungus to Promote Growth and Nutrient Management in Cucumber Plants vol.43, pp.1, 2013, https://doi.org/10.5941/myco.2015.43.1.49
  6. Some isolates of the nematophagous fungus Pochonia chlamydosporia promote root growth and reduce flowering time of tomato vol.166, pp.3, 2013, https://doi.org/10.1111/aab.12199
  7. Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms vol.10, pp.8, 2013, https://doi.org/10.1080/15592324.2015.1048052
  8. Enterobacter asburiae KE17 association regulates physiological changes and mitigates the toxic effects of heavy metals in soybean vol.17, pp.5, 2013, https://doi.org/10.1111/plb.12341
  9. The effect of Penicillium bilaii on wheat growth and phosphorus uptake as affected by soil pH, soil P and application of sewage sludge vol.3, pp.None, 2013, https://doi.org/10.1186/s40538-016-0075-3
  10. Foliar Treatment of Bacillus Methylotrophicus KE2 Reprograms Endogenous Functional Chemicals in Sesame to Improve Plant Health vol.57, pp.4, 2013, https://doi.org/10.1007/s12088-017-0666-0
  11. Plant defense approach of Bacillus subtilis (BERA 71) against Macrophomina phaseolina (Tassi) Goid in mung bean vol.12, pp.1, 2013, https://doi.org/10.1080/17429145.2017.1373871
  12. Bacillus : A Biological Tool for Crop Improvement through Bio-Molecular Changes in Adverse Environments vol.8, pp.None, 2013, https://doi.org/10.3389/fphys.2017.00667
  13. Fungal Diversity and Community Composition of Culturable Fungi in Stanhopea trigrina Cast Gibberellin Producers vol.9, pp.None, 2018, https://doi.org/10.3389/fmicb.2018.00612
  14. Isolation and Characterization of Endophytic Fungi from Purslane and the Effects of Isolates on the Growth of the Host vol.9, pp.5, 2013, https://doi.org/10.4236/aim.2019.95026
  15. Titanium dioxide and zinc oxide nanoparticles affect some bacterial diseases, and growth and physiological changes of beetroot vol.25, pp.5, 2019, https://doi.org/10.1080/19315260.2018.1523267
  16. The plant growth‐promoting potential of the mesophilic wood‐rot mushroom Pleurotus pulmonarius vol.127, pp.4, 2013, https://doi.org/10.1111/jam.14375
  17. Auxins of microbial origin and their use in agriculture vol.104, pp.20, 2020, https://doi.org/10.1007/s00253-020-10890-8
  18. Performance of tomato and lettuce to arbuscular mycorrhizal fungi and Penicillium pinophilum EU0013 inoculation varies with soil, culture media of inoculum, and fungal consortium composition vol.16, pp.None, 2013, https://doi.org/10.1016/j.rhisph.2020.100246
  19. Biological control of Fusarium wilt of sesame by Penicillium bilaiae 47M-1 vol.158, pp.None, 2013, https://doi.org/10.1016/j.biocontrol.2021.104601
  20. Alleviating salt stress on seedings using plant growth promoting rhizobacteria isolated from the rhizosphere soil of Achnatherum inebrians infected with Epichloë gansuensis endophyte vol.465, pp.1, 2013, https://doi.org/10.1007/s11104-021-05002-y
  21. Plant growth-promoting ability and control efficacy of Penicillium aurantiogriseum 44M-3 against sesame Fusarium wilt disease vol.31, pp.12, 2021, https://doi.org/10.1080/09583157.2021.1946011