Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Water Temperature and Salinity on Blood Properties and Oxygen Consumption in Hagfish (Eptatretus burgeri)

먹장어(Eptatretus burgeri)의 혈액성상과 산소소비에 미치는 수온 및 염분의 영향

  • Received : 2013.12.09
  • Accepted : 2014.02.13
  • Published : 2014.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Hagfish Eptatretus burgeri is classified as a agnathans and has many different physiological properties compared with vertebrates. In this study, we examined effects of water temperature and salinity on blood properties and oxygen consumption in hagfish. In the experiment of water temperature change, hematocrit (Ht), red blood cell (RBC) and glucose of hagfish blood revealed the lowest values at $15^{\circ}C$. Oxygen consumption of hagfish had significantly increased with rising water temperature, and the increasing rate was twice as much when the temperature was manipulated every $5^{\circ}C$. Also, oxygen consumption during the night time (a short photoperiod) was significantly higher than that of the daytime. Q10 level was 3.50 in the light period and 3.92 in the dark period. No significant change in plasma glucose level was showned in changing salinity from 30 psu to 22 psu, while it had rapidly increased at 20 psu ($13.7{\pm}4.0mg/dL$) and thereafter all hagfish were dead at 18 psu. However, osmolarity, $Na^+$, $K^+$ and $Cl^-$ levels had significantly decreased when salinity decreased. This results are expected to develop the artificial rearing techniques of natural hagfish.

Keywords

References

  1. Byun, Soon-Gyu..Jeong, Min-Hwan..Lee, Jong-Ha..Lee, Bea-Ik..Ku, Hag-Dong..Park, Sang-Un..Kim, Yi-Cheong & Chang, Young Jin (2008). Diel rhythm of oxygen consumption of the starry flounder Platichthys stellatus by water temperature, Journal of Korean Fisheries Society 41(2), 113-118. https://doi.org/10.5657/kfas.2008.41.2.113
  2. Chang, Young Jin..Hur, Jun-Wook & Lim, Han-Kyu (2001). Growth and survival of juvenile grey mullet, Mugil cephalus in rearing system with recirculated seawater and freshwater, Journal of Aquaculture 14(1), 29-33.
  3. Davis, K. B. & Parker, N. C. (1990). Physiological stress in striped bass: Effect of acclimation temperature. Aquaculture 91, 349-358. https://doi.org/10.1016/0044-8486(90)90199-W
  4. Fudge, D. S..Levy, N..Chiu, S. & Gosline, J. M. (2005). Composition, morphology, mechanics of hagfish slime, Journal of Experimental Biology 208, 4613-4625. https://doi.org/10.1242/jeb.01963
  5. Go, Hye-Jin (2000). Purification of bio-active peptide from hagfish, Eptatretus burgeri. Master thesis. Pukyung National University, Busan, Korea.
  6. Hwang, Eun-Young (1999). Nutritional qualities of meat protein and biologically active peptide in hagfish, Eptatretus burgeri. Ph.D. thesis. Pukyung National University, Busan, Korea.
  7. Jeong, Min-Hwan..Kim, Young-Soo..Min, Byung-Hwa & Chang, Young Jin (2007). Effect of fish number in respiratory chamber on routine oxygen consumption of black porgy, Acanthopagrus schlegeli reared in seawater or freshwater, Journal of Aquaculture 20(2), 121-126.
  8. Josef, D. V..Villani, P..Gasteiger, E. & Niederstatter, H. (1998). Oxygen consumption in sea bass fingerling, Dicentrachus labrax exposed to acute salinity and temperature changes: Metabolic basis for maximum stocking density estimations. Aquaculture 169, 303-313. https://doi.org/10.1016/S0044-8486(98)00375-5
  9. Kim, Il-Nam..Chang, Young Jin & Kwon, Jun-Yeong (1995). The patterns of oxygen consumption in six species of marine fish, Journal of Korean Fisheries Society 28(3), 313-381.
  10. Lim, J..Fudge, D. S..Levy, N. & Gosline, J. M. (2006). Hagfish slime ecomechanics: testing the gill-clogging hypothesis, Journal of Experimental Biology 209, 702-710. https://doi.org/10.1242/jeb.02067
  11. Morisawa, S. (1999). Fine structure of micropylar region during late oogenesis in egg of the hagfish Eptatretus burgeri (Agnatha), Development Growth & Differentiation 41, 611-618. https://doi.org/10.1046/j.1440-169x.1999.00458.x
  12. Morisawa, S. (2005). Spermiogenesis in the hagfish Eptatretus burgeri, Biological Bulletin 209, 204-214. https://doi.org/10.2307/3593110
  13. Morisawa, S. & Cherr, G. N. (2002). Acrosome reaction in spermatozoa from hagfish (Agnatha) Eptatretus burgeri and Eptatretus stouti: Acrosomal exocytosis and identification of filamentous actin, Development Growth & Differentiation 44, 337-344. https://doi.org/10.1046/j.1440-169X.2002.00643.x
  14. Nozaki, M..Ichikawa, T..Tsuneki, K. & Kobayashi, H. (2000). Seasonal development of gonads of the hagfish Eptatretus burgeri correlated with their seasonal migration. Zoological Science 17, 225-232. https://doi.org/10.2108/zsj.17.225
  15. Ooka-Souda, S..Kadota, T. & Takeuchi, H. (2000). Circadian rhythms in locomotor activity of the hagfish, Eptatretus burgeri, The effect of cutting the spinal cord. Zoological Science 17, 329-333.
  16. Shin, Mi-Jung.Kim, Eun-Jung.Kim, Chan-Hee.Go, Hye-Jin.Kim, In-Hae.Ryu, Hong-Soo.Huh, Min-Do.Chung, Joon-Ki & Park, Nam-Gyu (2003). Purification of a bradykinin-related peptide from the skin of hagfish, Eptatretus burgeri, Journal of Korean Fisheries Society 36(1), 30-34. https://doi.org/10.5657/kfas.2003.36.1.030
  17. Sohn, Hee-Young (2006). cDNA cloning of bradykinin-related peptide isolated from Korean hagfish, Eptatretus burgeri. Master thesis, Pukyung National University, Busan, Korea.