Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-diabetic effect of the mixture of mulberry leaf and green tea powder in rats with streptozotocin-induced diabetes

뽕잎과 녹차혼합분말의 급여가 streptozotocin 유발 당뇨쥐의 항당뇨효과

  • Son, Hee-Kyoung (Department of Food and Nutrition, Chosun University) ;
  • Han, Ju-Hee (Department of Food and Nutrition, Chosun University) ;
  • Lee, Jae-Joon (Department of Food and Nutrition, Chosun University)
  • 손희경 (조선대학교 식품영양학과) ;
  • 한주희 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2014.04.28
  • Accepted : 2014.05.22
  • Published : 2014.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the hypoglycemic effects of mulberry leaf (M), green tea (G), and a mixture of mulberry leaf and green tea (MG) in rats with streptozotocin-induced diabetes. Male Sprague-Dawley rats were divided into the non-diabetic (N) and diabetic groups. The experimental animals were divided into four groups and fed the following for four weeks: a normal diet (N), the STZ+normal diet (STZ), the STZ+5% mulberry leaf (STZ-M), STZ+5% green tea (STZ-G), and the STZ+5% mixture of mulberry leaf and green tea (STZ-MG). The whole blood glucose level of the STZ-M, STZ-G and STZ-MG groups significantly decreased compared to the STZ group two weeks later. The serum glucose and fructosamine, and urine glucose levels in the STZ-M, STZ-G and STZ-MG groups were lower than in the STZ group. The levels of serum insulin were higher in the STZ-M, STZ-G and STZ-MG groups than in the STZ group. However, serum glucose, fructosamine and insulin levels, and urine glucose levels were not significantly different among the STZ-M, STZ-G and STZ-MG groups. These results indicate that mulberry leaf, green tea, and their mixture help prevent or attenuate the progression of diabetes in rats with STZ-induced diabetes.

뽕잎, 녹차 및 뽕잎과 녹차혼합물이 sterptozotocin 유발 당뇨 쥐의 혈당 조절효과에 대해 살펴보았다. 실험군은 정상군(N), 당뇨 대조군(STZ), 5% 뽕잎 분말 첨가 당뇨군(STZ-M), 5% 녹차 분말 첨가 당뇨군(STZ-G) 및 2.5% 뽕잎과 2.5% 녹차혼합분말 첨가 당뇨군(STZ-MG)으로 5군으로 나누어 4주간 실시하였다. 체중증가량은 뽕잎, 녹차 및 뽕잎과 녹차혼합물 첨가 당뇨군(STZ-M, STG-G, STZ-MG)들이 당뇨 대조군(STZ)에 비하여 유의적 차이는 없었으나 다소 증가하였다. 뇨 중 포도당 함량은 뽕잎, 녹차 및 뽕잎과 녹차혼합물 첨가 당뇨군(STG-G, STZ-MG)들이 당뇨 대조군(STZ)에 비하여 감소효과를 보였다. 4주간 혈당의 변화는 뽕잎, 녹차 및 뽕잎과 녹차혼합물(STZ-M, STG-G, STZ-MG)의 급여로 혈당 수준이 감소하는 경향이었고, 뽕잎과 녹차혼합물 첨가 당뇨군(STZ-MG)이 혈당 강하효과가 가장 높았다. 시험 종료 후 혈청 포도당 함량은 뽕잎, 녹차 및 뽕잎과 녹차혼합물 첨가(STZ-M, STG-G, STZ-MG)로 당뇨 대조군(STZ)에 비하여 모두 유의적으로 낮게 나타나 정상군(N)과 비슷한 경향을 보였다. 혈청 인슐린 함량은 녹차 및 뽕잎과 녹차혼합물 첨가(STG-G, STZ-MG)로 당뇨 대조군(STZ)에 비하여 유의적으로 증가하였고, 혈청 fructosamine 함량은 유의적으로 감소하였다. 이상의 결과 뽕잎, 녹차 및 뽕잎과 녹차혼합물의 급여는 STZ로 유발된 당뇨 쥐의 혈당 강하효과를 확인하여, 당뇨성 질환의 예방 및 개선에 효과가 있을 것으로 사료된다.

Keywords

References

  1. Lee SJ, Park JY, Nam CM, Jee SH (2008) The prevalence estimation of metabolic syndrome and it's related factors based on data from general health medical examination: a multi-center study. J Korean Soc Health Information Health Statistics, 33, 119-133
  2. Statistics Korea (2013) Annual report on the cause of 2012 death statistics. Korean National Statistical Office, Daejeon, Korea
  3. Reaven GM (1988) Tole of insulin resistance in human disease. Diabetes, 37, 1597-1607.
  4. O'Brien RM, Granner DK (1996) Regulation of gene expression by insulin. Physiol Rev, 76, 1109-1161
  5. Hayden JM, Reaven PD (2000) Cardiovascular disease in diabetes mellitus type 2: a potential role for novel cardiovascular risk factors. Curr Opin Lipidol, 11, 519-528 https://doi.org/10.1097/00041433-200010000-00010
  6. Bailey CJ (1999) Insulin resistance and antidiabetic drugs. Biochem Pharmacol, 58, 1511-1520 https://doi.org/10.1016/S0006-2952(99)00191-4
  7. Jeon YS, Kim MW (2011) The antioxidative effects and isolation and characterization of the extracts from Morus alba L. Korean J Food Nutr, 24, 94-100 https://doi.org/10.9799/ksfan.2011.24.1.094
  8. Courtiere A, Molard F, Reyband J (1995) Differential effects of in vitro peroxidation on peripheral- and central-type benzodiazepine receptors. Protection by diverse antioxidants. Biochem Pharmacol, 50, 181-182 https://doi.org/10.1016/0091-3057(94)00282-N
  9. Chung SH, Yu JH, Kim EJ, Ryu KS (1996) Blood glucose lowering effect of silkworm. Bull K H Pharma Sci, 24, 95-100
  10. Massayasu K, Fu-jun Chen, Noboru N, Ikuho K, Naoki A, Sakuji K (1995) Anti hyperglycemic effect of N-containing sugars delived from mulberry leaves in streptozotocin-induced diabetic mice. J Traditional Medicine, 12, 214-219
  11. Kim SY, Ryu KS, Lee WC, Ku HO, Lee HS, Lee KR (1999) Hypoglycemic effect of mulberry leaf with anaerobic treatment in alloxan-induced diabetic mice. Korean J Pharmacol 30, 123-129
  12. Choi HK, Oh KH (2012) Cellular responses of Salmonella typhimurium exposed to green tea polyphenols. Korean J Microbiol, 48, 87-92 https://doi.org/10.7845/kjm.2012.48.2.087
  13. Choi GN, Jeong CH, Kim JH, Kwak JH, Shin YH, Lee SC, Cho SH, Choi SG, Heo HJ (2009) Effect of storage temperature and water activity on antioxidant activities of powdered green tea extracts. Korean J Food Preserv, 16, 333-341
  14. Hyon SH, Kim DH (2001) Long-term preservation of rat pancreatic islets under physiological conditions. J Biotechnol, 85, 241-246 https://doi.org/10.1016/S0168-1656(00)00336-9
  15. Rhee SJ. Park GY (1997) Effects of green tea catechin on liver 3-hydroxy-3-methylglutaryl Co enzyme a reductase activity and serum lipid levels in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr, 26, 1187-1193
  16. Wolfram S, Raederstorff D, Preller M, Wang Y, Teixeira SR, Riegger C, Weber P (2006) Epigallocatechin gallate supplementation alleviates diabetes in rodents. J Nutr, 136, 2512-2518
  17. Friedwald W, Levy R, Fredrickson D (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem, 18, 499-502
  18. Johnson RN, Metcalf PA, Baker JR (1982) Fructosamine : a new approach to the estimation of serum glycosylprotein. An index of diabetic control. Clinica Chimia Acta, 127, 87-95
  19. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Tuner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia, 28, 412-419 https://doi.org/10.1007/BF00280883
  20. Folch J, Lees M, Sloane-Stanley G (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem, 226, 497-509
  21. Zlatkis A, B Zak (1969) Study of a new cholesterol reagent. Anal Biochem, 29, 143-148 https://doi.org/10.1016/0003-2697(69)90017-7
  22. Biggs HG, Erikson TM, Moorehead WR (1975) A mamual colirimetric assay of triglyceride in serum. Clinical Chem, 21, 437-438.
  23. Cho YJ, Bang MA (2004) Hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocin-induced diabetic rats. Korean J Nutr, 37, 5-14
  24. Kim SH (2008) Effects of green tea a powder on plasma and liver lipid concentration in diabetic rats. MS Thesis, Keimyung University, Daegu, Korea.
  25. Hong JH (2002) Effects of YK-209 mulberry leaves on improvement of lipid metabolism in streptozotocin induced diabetic rats. MS Thesis, Catholic University of Daegu, Korea.
  26. Park SA, Kim MJ, Jang JY, Choi MS, Yeo J, Lee MK (2006) Effect of genistein and daidzein on antioxidant defense system in C57-BL/KsJ-db/db mice. J Korean Soc Food Sci Nutr, 35, 1159-1165 https://doi.org/10.3746/jkfn.2006.35.9.1159
  27. Dai S, Thompson KH, McNeill JH (1994) One-year treatment of streptozotocin-induced diabetic rats with vanadyl sulphate. Pharmacol Toxicol, 74, 101-109 https://doi.org/10.1111/j.1600-0773.1994.tb01083.x
  28. Seyer-Hansen K (1977) Renal hypertrophy in experimental diabetes: relation to severity of diabetes. Diabetologia, 13, 141-143 https://doi.org/10.1007/BF00745142
  29. Steer KA, Sochor M, McLean P (1985) Renal hypertrophy in experimental diabetes changes in pentose phosphate pathway activity. Diabetes, 34, 485-490 https://doi.org/10.2337/diab.34.5.485
  30. Rhee SJ, Choi JH (2001) Effects of green tea catechin on the superoxide dismutase, glutathione peroxidase and xanthine oxidase activites of kidney in diabetic rats. J Nutr Hlth, 34, 734-740
  31. Beppu H, Maruta K, Kurner T, Kolb H (1987) Diabetogenic action of streptozotocin: essential role of membrane permeability. Act Endocrinol (Copdn), 114, 90-96
  32. Smith OLK, Wong CY, Gelfand RA (1989) Skeletal muscle proteolysis in rat with acute streptozotocininduced diabetes. Diabetes, 38, 1117-1122 https://doi.org/10.2337/diab.38.9.1117
  33. Park CO, Jin SH, Ryu BH (1996) Antioxidant activity of green tea extracts toward human low density lipoprotein. Korean J Food Sci Technol, 28, 850-858
  34. Matkovics B, Kotorman M, Varga C (1998) Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung, 85, 29-38
  35. Like AA, Appe MC, Rossin AA (1982) Autoantibodies in the BB/W rat. Diabetes, 31, 816-820 https://doi.org/10.2337/diab.31.9.816
  36. Lee SY, Park SL, Nam YD, Yi SH, Lim SI (2013) Anti-diabetic effects of fermented green tea in KK-Ay diabetic mice. Korean J Food Sci Technol, 45, 488-494 https://doi.org/10.9721/KJFST.2013.45.4.488
  37. Kamiyama O, Sanae F, Ikeda K, Higashi Y, Minami Y, Asano N, Adachi I, Kato A (2010) In vitro inhibition of ${\alpha}$-glucosidases and glycogen phosphorylase by catechin gallates in green tea. Food Chem, 122, 1061-1066 https://doi.org/10.1016/j.foodchem.2010.03.075
  38. Anderson RA, Polansky MM (2002) Tea enhances insulin activity. J Agr Food Chem, 50, 7182-7186 https://doi.org/10.1021/jf020514c
  39. Yoo SK, Kim MJ, Kim JW, Rhee SJ (2002) Effects of YK-209 mulberry leaves on disaccharidase activites of small intestine and blood glucose-lowering in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr, 31, 1071-1077 https://doi.org/10.3746/jkfn.2002.31.6.1071
  40. Park JH, Lee KW, Sung KS, Kim SS, Cho KD, Lee BH, Han CK (2012) Effect of diets with mulberry leaf and Cudrania tricuspidata leaf powder supplements on blood glucose-related biomarkers in streptozotocininduced diabetic rats. J Korean Soc Food Sci Nutr, 41, 766-773 https://doi.org/10.3746/jkfn.2012.41.6.766
  41. Di Mauro, M., D. Lazzarini, P. Fumelli, F. Carle, A. Kosmidis (2007) Bioelectrical impedance analysis and diabetes mellitus: which correlation among fructosamine, glycosylated haemoglobin and exchangeable potassium. Minerva Med, 98, 633-638
  42. Choi JW, Sohn KH, Kim SH (1991) Effects of nicotinamide on the serum lipid composition in streptozotocin-induced diabetic rats. J Korean Soc Food Nutr, 20, 306-311
  43. O'Meara NM, Devery RA, Owens D, Collins PB, Johnson AH, Tomkin GH (1990) Cholesterol metabolism in alloxan induced diabetic rabbits. Diabetes, 39, 629-633
  44. Goren T, Castelli WP, Hjorland MC, Kannel WB, Dawber TR (1997) High density lipoprotein as a protective factor against coronary heart disease: The framingham study. Am J Med, 62, 707-714
  45. Ganda OP (1985) Pathogenesis of macrovascular disease including in the influence of lipid in : Joslins' Diabetes Mellitus, 12th edition, Lea & Febiger. FA, USA, P 217
  46. Kahn CR (1985) The molecular mechanism of insulin action. Ann Rec Med, 36, 249-251
  47. Nikkila EA, Kekki M (1973) Plasma triglyceride transport kinetics in diabetes mellitus. Metabolism, 22, 1-22 https://doi.org/10.1016/0026-0495(73)90024-3
  48. Cha JY, Kim HJ, Jeon BS, Jo YS (2000) Effect of water-extracted of leaves from Morus alba and Cudrania tricuspidata on the lipid concentrations of serum and liver in rats. J Korean Soc Agric Chem Biotechnol, 43, 303-308

Cited by

  1. Development of Bitter Melon Juice Mixed with Condensed Oat Juice and Its Hypoglycemic Effect on Streptozotocin-induced Diabetic Rats vol.25, pp.2, 2016, https://doi.org/10.5934/kjhe.2016.25.2.227
  2. The Efficacy of Lowering Blood Glucose Levels Using the Extracts of Fermented Bitter Melon in the Diabetic Mice vol.58, pp.3, 2015, https://doi.org/10.3839/jabc.2015.041
  3. 명월초, 여주 및 울금을 포함하는 돼지감자 복합물의 streptozotocin 유발 당뇨쥐에서 혈당강하 및 체내 지질개선에 미치는 영향 vol.28, pp.6, 2018, https://doi.org/10.5352/jls.2018.28.6.671
  4. Quality Characteristics of Inner Beauty Foods (Mook) Prepared with Mixture of Mulberry Leaf and Fruit Powder vol.16, pp.4, 2014, https://doi.org/10.20402/ajbc.2018.0238
  5. Leuconostoc mesenteroidesies 균주를 이용한 여주 추출물 발효 및 생산물의 생리활성 특성 vol.35, pp.4, 2014, https://doi.org/10.12925/jkocs.2018.35.4.1250