Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant and antimicrobial activity of solvent fractions from black bamboo leaves

오죽 잎 용매분획물의 항산화 및 항균 활성

  • Bae, Hyun-Kyung (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Chung, Shin-Kyo (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2014.05.26
  • Accepted : 2014.07.15
  • Published : 2014.08.30
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Abstract

To investigate the natural antioxidant and antimicrobial phytochemicals from black bamboo (Phyllostachys nigra MUNRO) leaves, the solvent fractions from crude methanol extract were made with n-hexane, ethyl acetate, and butanol, and their antioxidant and antimicrobial activities were determined. The antioxidant activities were examined by 1,1-diphenyl-1-picrylhydrazyl (DPPH) method and ferric ion reducing antioxidant power (FRAP) method, and the antimicrobial activities against Staphylococcus aureus were tested by paper disc agar diffusion method. Total phenolic contents and total flavonoid contents of the solvent fractions were also determined. The ethyl acetate fraction with the highest total phenolic contents among all fractions showed the strong antioxidant activities by DPPH method and FRAP method, and antimicrobial activities against S. aureus at all test concentrations. Caffeic acid, ferulic acid, quercetin, and kaempferol were analyzed by HPLC in the ethyl acetate fraction from black bamboo leaves by the comparison with the standard chemicals. It is supposed that the ethyl acetate fraction from black bamboo leaves could be used as natural preservatives in the food industry.

오죽 잎의 메탄올 조추출물을 극성에 따라 용매분획하고 분획물의 항산화 활성과 항산화 활성, S. aureus 균주에 대한 항균활성을 조사하고 활성성분을 HPLC를 이용하여 정량하였다. 용매분획물 중에서 ethyl acetate 분획물의 DPPH radical 소거 활성, FRAP활성이 모두 가장 강하였으며, 총페놀성 화합물의 함량도 가장 높았다. S. aureus 균주에 대한 항균활성 역시 ethyl acetate 분획물이 모든 시험 농도에서 가장 높은 활성을 보였다. Ethyl acetate 분획물 중에서 caffeic acid, ferulic acid, quercetin, kaempferol 성분을 분리하여 정량하였으며 이 중 kaempferol의 함량이 가장 높았다. 오죽 잎의 ethyl acetate 분획물은 천연 식품보존료로서 활용이 가능할 것으로 보인다.

Keywords

References

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