DOI QR코드

DOI QR Code

Effect of Light Emitting Diode Irradiation on Tyrosinase Activity during Storage

저장 중 발광다이오드 광 조사가 타이로시네이스 활성에 미치는 영향

  • Jung, Hyo Yeon (Department of Food Science and Technology, Seoul Women's University) ;
  • Kim, Jung Sun (Department of Food Science and Technology, Seoul Women's University) ;
  • Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University)
  • 정효연 (서울여자대학교 식품공학과) ;
  • 김정선 (서울여자대학교 식품공학과) ;
  • 노봉수 (서울여자대학교 식품공학과)
  • Received : 2015.08.27
  • Accepted : 2015.10.09
  • Published : 2015.12.31

Abstract

In this study, the effect of light irradiation on tyrosinase activity during storage in a low temperature incubator was investigated, with the goal of identifying the appropriate wavelength and intensity of light for inhibiting tyrosinase activity. Fluorescent light and blue light emitting diode (LED) irradiation were found to inhibit tyrosinase activity. In particular, tyrosinase activity was significantly inhibited under high intensity blue LED irradiation, suggesting that the activity of tyrosinase present in vegetables or fruits might be inhibited by LED irradiation. Therefore, blue LED irradiation during food storage and the distribution stage would be useful for ensuring good quality of food, owing to the inhibition of the enzymatic browning reaction.

식품 저장 중 효소적 갈변을 일으키는 타이로시네이스의 활성을 저해시킬 수 있는 LED의 파장을 구명하기 위하여 파란색, 초록색, 빨간색 LED와 형광등을 이용하여 타이로시네이스에 7일동안 조사하였다. 또한 강도에 따른 타이로시네이스의 활성을 조사하기 위하여 파란색, 초록색, 빨간색 LED를 선택하여 빛의 강도를 3단계로 구분하여 사용하였다. 파장에 따른 타이로시네이스의 활성 저해율은 형광등에서 가장 컸으며 그 다음으로 파란색 LED에서 높은 저해율을 보였다. 빨간색 LED에서는 대조구와 유사한 저해율을 보였으며 초록색 LED에서 저해율이 가장 낮았다. 상대적 빛의 강도에 따른 타이로시네이스 활성은 초록색과 빨간색 LED 조사 시 효소활성의 변화가 크지 않았고, 반면 파란색 LED에서 강도가 증가할수록 큰 저해효과를 보였다. 이러한 결과를 토대로 볼 때 여러 가지 파장의 LED 중 고강도의 파란색 LED에서 타이로시네이스에 의한 식품의 갈변을 최소화 할 수 있을 것으로 판단되며 이는 갈변 억제를 유도함으로써 과일 및 채소의 저장 중 품질을 향상시킬 수 있을 것이다.

Keywords

References

  1. Kwak JH, Kim YH, Chang HR, Park CW, Han YH. Inhibitory effect of gardenia fruit extracts on tyrosinase activity and melanogenesis. Korean Soc. Biotechnol. Bioeng. 19: 437-440 (2004)
  2. Lee KH, Cho HS, Kim DS, Hong BI, Park CS, Kim MG. Browning of ascidian meat, Halocynthia roretzi and its prevention. Korean J. Fish. Aquat. Sci. 26: 214-220 (1993)
  3. Kim MJ, Kim CY, Park IS. Prevention of enzymatic browning of pear by onion extract. Food Chem. 89: 181-184 (2005) https://doi.org/10.1016/j.foodchem.2004.02.018
  4. Mun KZ. Study on tyrosinase inactivation using high-voltage pulsed electric field. MS thesis, Yonsei University, Seoul, Korea (2001)
  5. Choi HG, Kwon JK, Moon BY, Kang NJ, Park KS, Cho MW, Kim YC. Effect of different light emitting diode (LED) lights on the growth characteristics and the phytochemical production of strawberry fruits during cultivation. Korean J. Hort. Sci. Technol. 31: 56-64 (2013) https://doi.org/10.7235/hort.2013.12100
  6. Kim TS, Lee SP, Park SI, Lee JY, Lee SY, Jun HJ. Physicochemical properties of broccoli sprouts cultivated in a plant factory system with different lighting conditions. J. Korean Soc. Food Sci. Nutr. 40: 1757-1763 (2011) https://doi.org/10.3746/jkfn.2011.40.12.1757
  7. Kim NY, Lee DS, Lee HJ, An DS. Effect of UV-LED irradiation on respiration and ethylene production of cherry tomatoes. Korean J. Food Preserv. 19: 47-53 (2012) https://doi.org/10.11002/kjfp.2012.19.1.047
  8. Baek KH, Jang MH, Kwack YB, Lee SW, Yun HK. Regulation of acid contents in kiwifruit irradiated by various wavelength of light emitting diode during postharvest storage. Clean Technol. 16: 88-94 (2010)
  9. Park IS, Choi DJ, Youn AR, Lee YJ, Kim YK, Kim MH, Choi SR, Kim KH, Dong HM, Han HJ, Noh BS. Effect of light emitting diode and fluorescent light on volatile profiles of soybean oil during storage. Korean J. Food Sci. Technol. 45: 763-769 (2013) https://doi.org/10.9721/KJFST.2013.45.6.763
  10. Park SY, Chang MS, Choi JH, Kim BS, Lee HR, Ham KH. Effect of a refrigerator with LED on functional composition changes and freshness prolongation of cabbage. Korean J. Food Preserv. 14: 113-118 (2007)
  11. Ishihara Y, Oka M, Tsunakawa M, Tomita K, Hatori M, Yamamoto H, Kamei H, Miyaki T, Konishi M, Oki T. Melanostatin, a new melanin synthesis inhibitor: Production, isolation, chemical properties, structure and biological activity. J. Antibiot. 44: 25-32 (1991) https://doi.org/10.7164/antibiotics.44.25
  12. Yang DC, Choi HY, Kim YH, Yun KY, Yang DC. Growth and ginsenosides production of hairy root (Panax ginseng C.A. Meyer) via light energy. J. Ginseng Res. 20: 318-324 (1996)
  13. Kim HJ. Effect of wavelength on the enzyme activity and its storage stability. MS thesis, Seoul National University of Science and Technology, Seoul, Korea (2000)
  14. Yang DC, Kim YH, Kwon JN, Choi CH, Yang DC. Effects of light on activities of antioxidative enzymes in hairy rood cultures of Phytolacca esculenta van houtte. J. Plant Biotechnol. 22: 71-76 (1995)
  15. Jung K. Effects of light-emitting diode (LED) irradiation on the $H_{2}O_{2}$-induced senescent cells. MS thesis, Chonnam National University, Gwangju, Korea (2006)