Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Dropwort (Oenanthe javanica) Extracts on Memory Improvement in Alzheimer's Disease Animal Model, Tg2576 mice

알츠하이머질병 모델동물인 Tg2576 마우스를 이용한 미나리 알코올추출물의 기억력 개선 효능

  • Won, Beom Young (Research & Development Center, Braintropia Co., Ltd.) ;
  • Shin, Ki Young (Department of Microbiology, College of Natural Science, Dankook University) ;
  • Ha, Hyun Jee (Research & Development Center, Braintropia Co., Ltd.) ;
  • Chang, Keun-A (Department of Pharmacology, Gachon University of Medicine and Science) ;
  • Yun, Yeo Sang (Research & Development Center, Braintropia Co., Ltd.) ;
  • Kim, Ye Ri (Research & Development Center, Braintropia Co., Ltd.) ;
  • Park, Yong Jin (Research & Development Center, Braintropia Co., Ltd.) ;
  • Lee, Hyung Gun (Research & Development Center, Braintropia Co., Ltd.)
  • 원범영 ((주)브레인트로피아 기업부설연구소) ;
  • 신기영 (단국대학교 자연과학대학 미생물학과) ;
  • 하현지 ((주)브레인트로피아 기업부설연구소) ;
  • 장근아 (가천대학교 메디컬캠퍼스 약리학과) ;
  • 윤여상 ((주)브레인트로피아 기업부설연구소) ;
  • 김예리 ((주)브레인트로피아 기업부설연구소) ;
  • 박용진 ((주)브레인트로피아 기업부설연구소) ;
  • 이형근 ((주)브레인트로피아 기업부설연구소)
  • Received : 2015.10.12
  • Accepted : 2015.12.10
  • Published : 2015.12.31
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Abstract

This study was conducted to investigate the effect of herbs on memory improvement by focusing on their cholinergic functions in Tg2576 mice. Seven herbs were used to obtain extracts by using alcohol and water. In screening test for cholinergic activities of the extracts, acetylcholinesterase (AChE) activity was highly inhibited in Oenanthe javanica alcohol extract (OJAE, 18.76%) as compared with the others. The OJAE-treated Tg2576 (Tg-OJAE) groups showed the statistically significant increases of latency time in passive avoidance test. Also, it was found that the concentration of $A{\beta}1-42$ was significantly reduced in Tg-OJAE groups compared to non-treated Tg2576 groups. In the additional enzyme test, it was found that $IC_{50}$ of OJAE was $991.77{\mu}g/mL$ and OJAE acted as an uncompetitive inhibitor of AChE. Therefore, it seemed that OJAE can be used for the development of processed foods for memory improvement.

본 연구는 천연 식물이 기억력 개선에 미치는 영향을 검토하기 위하여 총 7가지 식물에 대하여 아세틸콜린분해효소 활성 억제력을 측정하였다. 특히 미나리 알코올추출물(18.76%)의 억제력이 가장 우수하였으며, 미나리 알코올추출물에 대한 추가 연구를 수행하였다. Tg2576 마우스의 기억력에 미치는 영향을 검토하기 위하여 미나리 알코올추출물 50 mg/kg으로 3개월간 경구투여 후 수동회피테스트로 인지기능변화를 측정하였고, 뇌 속의 아세틸콜린분해효소 활성 억제, 베타아밀로이드1-42 단백질 생성 억제력을 측정하였다. 그 결과, 수동회피 테스트에서 미나리추출물을 투여한 Tg 마우스군은 181.77초로 생리식염수를 투여한 Tg 마우스군과 비교하여 머무름 시간이 유의적으로 증가하게 나타났다. 베타아밀로이드1-42 단백질 농도 측정 시 미나리 알코올추출물에 의하여 축적 농도가 985.19 pmol/g으로 감소하였으며, 생리식염수를 투여한 Tg 마우스군과 유의적 차이가 있었다. 추가적인 효소 억제력 실험 결과, 미나리 알코올추출물의 아세틸콜린분해효소 활성억제에 대한 50% 활성억제농도($IC_{50}$)값은 $991.77{\mu}g/mL$로 나타났으며, Lineweaver-Burk Plot 결과, 무경쟁적 저해로 나타났다. 따라서 미나리 알코올추출물은 Tg2576 형질전환 마우스의 인지기능을 개선시키며, 콜린성 신경시스템을 보호하는 물질로 판단된다. 미나리 알코올추출물은 기억 및 학습 증진에 효과적으로 작용하는 천연식물로써 이상의 결과를 근거로 한 미나리 소재의 다양한 기능성제품 개발과 부가가치 향상이 가능할 것으로 판단된다.

Keywords

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