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식물공장에서 재배한 브로콜리 새싹 용매 분획물의 항산화 활성

Antioxidant Activity of Solvent Fraction from Broccoli Sprouts Cultivated at the Plant Factory System

  • 김은지 (호서대학교 생명보건과학대학 식품영양학과 및 기초과학연구소) ;
  • 김미혜 (호서대학교 생명보건과학대학 식품영양학과 및 기초과학연구소)
  • Kim, Eun-Ji (The Research Institute for Basic Sciences, Dept. of Food and Nutrition, Hoseo University) ;
  • Kim, Mi-Hye (The Research Institute for Basic Sciences, Dept. of Food and Nutrition, Hoseo University)
  • 투고 : 2013.10.14
  • 심사 : 2014.12.05
  • 발행 : 2015.02.28

초록

This study was designed to determine the antioxidant activity of solvent fractions of broccoli sprouts grown by controlling the growing environment at the plant factory system. Fractionation was achieved with chloroform, n-hexane, ethyl acetate, butanol, water by 70% EtOH extract of the broccoli sprouts. Each solvent fraction was put through TLC and HPLC to separate active components. Higher antioxidant activities were observed for the butanol and ethyl acetate layers. Further evaluation of each of the 5 layers (LH1 to LH5) of the butanol fraction showed that the refined LH3 extract had a high antioxidant effect. Components with similar Rf values from TLC had the same retention times and peaks in the HPLC analysis. It was also determined that the sulforaphane content was high at the chloroform and butanol layers and the sulforaphane was responsible for, the high antioxidant activity. Thus, to use for functional materials, the butanol extract/layer of broccoli sprouts is recommended as the most effective.

키워드

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피인용 문헌

  1. 브로콜리 새싹 용매 추출물의 항산화 및 면역조절 활성 vol.32, pp.1, 2019, https://doi.org/10.9799/ksfan.2019.32.1.001