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Nutritional Components of Korean Traditional Actinidia (Actinidia arguta) Sprout and in vitro Antioxidant Effect

한국 토종다래(Actinidia arguta)순의 주요 영양성분 및 in vitro 항산화 활성

  • Jin, Dong Eun (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Chang Hyeon (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seung, Tae Wan (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 진동은 (경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원) ;
  • 박창현 (경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원) ;
  • 승태완 (경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 Plus), 농업생명과학연구원)
  • Received : 2014.11.11
  • Accepted : 2014.12.26
  • Published : 2015.02.28

Abstract

Nutritional composition and in vitro antioxidant activities of the ethanol extract of Korean traditional actinidia (Actinidia arguta) sprouts of the Otumsense variety were investigated to examine the sproutsi nutritional value. The most abundant mineral, amino acid, and fatty acid were calcium, glutamic acid, and ${\alpha}$-linolenic acid, respectively. The major free sugar of Otumsense sprouts was sucrose. The level of vitamin C, a natural antioxidant, was highest among other vitamins examined. The amount of total polyphenol was highest in the 40% ethanol extract. The 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulphonic acid) radical scavenging activity of the 40% ethanol extract was about 94% at a concentration of $1,000{\mu}g/mL$. Malondialdehyde inhibition by the extract increased in a dose-dependent manner (from 0 to $100{\mu}g/mL$). Intracellular reactive oxygen species accumulation resulting from $H_2O_2$ treatment of PC12 cells significantly reduced when the 40% ethanol extract was present in the media compared to that in PC12 cells treated with $H_2O_2$ only.

토종다래 순의 무기질은 Ca, K, P 순으로 많았으며, 아미노산은 glutamic acid, aspartic acid가 많이 함유되어 있었다. 지방산 함량은 ${\alpha}$-linolenic acid와 linoleic acid 등의 불포화지방산 함유량이 높았으며, 유리당은 sucrose의 함량이 상대적으로 높았다. Vitamin B 그룹에서는 vitamin $B_6$가 17.99mg/100 g으로 가장 높았고, vitamin C와 ${\beta}$-carotene 함량은 각각 42.57mg/100 g, $390.69{\mu}g/100g$이었다. Total phenolics는 40% EtOH 추출물이 198.08 mg GAE/g으로 가장 높았으며, ABTS radical 소거활성 및 DPPH radical 소거 활성에서는 농도 의존적으로 radical 소거 활성을 보였다. 지질 과산화 억제활성(MDA contents)과 세포내 산화적 스트레스 생성 억제효과에서는 지질과산화를 막아주며 신경세포 손상을 일정 수준에서 억제해 주는 것으로 확인하였다. 본 연구 결과를 종합해볼 때, 상대적으로 높은 phenolics를 함유한 토종다래순은 우수한 영양학적인 구성과 in vitro antioxidant activities를 기초로 한 고부가가치 건강지향식품 소재로서의 활용가치가 높다고 판단된다.

Keywords

References

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