Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antidiabetic effect of ethanol extract on Codonopsis lanceolata root in streptozotocin induced diabetic rats

더덕 뿌리 에탄올 추출물이 streptozotocin으로 유발된 흰쥐의 항 당뇨효과

  • Kim, Ok-Kyung (Department of Food Science and Nutrition, Dae Jin University)
  • 김옥경 (대진대학교 자연과학대학 식품영양학과)
  • Received : 2016.05.09
  • Accepted : 2016.06.08
  • Published : 2016.06.30
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Abstract

This study was done to investigate the antidiabetic effect of ethanol extract from Codonopsis lanceolata root in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose 45mg/kg.b.w. dissolved in citrate buffer(pH4.5). The ethanol extract of Codonopsis lanceolata root was orally administrated once a day for 7 days. The contents of serum glucose, triglyceride(TG) and total cholesterol were significantly decreased(p<0.05) in Codonopsis lanceolata root treated group compared to the those of STZ-control group. Also the content of hepatic glycogen and activities of glucose-phosphate dehydrogenase(G-6-PDH) and glucokinase(GK) were significamtly increased(p<0.05). These results indicated that ethanol extract of Codonopsis lanceolata root would have antidiabetic effect in STZ-induced diabetic rats.

Streptozotocin(STZ)을 45mg/kg.b.w의 용량으로 흰쥐의 미정맥에 투여한 후 유발된 당뇨 흰쥐에게 1일 1회 7일간 1,000mg/kg의 용량으로 투여 후 glucose 함량과 당대사에 관여하는 효소인 glucose-6-phosphatase(G-6-Pase), glucose-6-phosphate dehydrogenase(G-6-PDH), glucokinase(GK) 활성과 glycogen 함량, triglyceride(TG), total cholesterol등의 지질대사에 관여하는 물질들을 측정한 결과 더덕 뿌리 에탄올 추출물 투여군이 glucose, TG, total cholesterol등의 함량과 G-6-Pase 활성의 유의적인 감소(p<0.05)를 나타내었으며 glycogen 함량과 G-6-PDH, GK의 활성이 유의적인 증가(p<0.05)를 나타내었다. 이와 같이 더덕뿌리 에탄올 추출물이 항당뇨 개선효과를 갖는 유효성분을 함유하고 있음을 알 수 있었다.

Keywords

References

  1. S. J. Moon, Korean disease and nutrition pattern. Korean J. Nutr. 29, 381(1996).
  2. Ministry of health and welfare. Korea centers for disease control and prevention. korea health statics (2013).
  3. V. A. Kovisito, Insulin therapy in type II diabetes. Diabetes care 16. 29 (1993). https://doi.org/10.2337/diacare.16.3.29
  4. C. M. Kim, M. H. Chung, Pharmacognostical studies on codonopsis lanceolata on lympocyte and clonal macrophage. Korean J. Pharmacog.6, 43 (1975).
  5. W. S. Hong, J. S. Lee, S. Y. Ko, Y. S. Choi, A study on the perception of Codnopsis lanceolata dishes. Korean J. Food cookery Sci. 22, 181 (2006).
  6. Lee. J. H. Immunostumulative effect of hot-water extract from Codonopsis lanceolata on lymphocyte and clonal macrophage. Korean J. Food Sci.Technol. 34, 732 (2002).
  7. S. D. Park, G. H. Lee, Y. S. Lee, Y. K. Kwon, J. H. Park, S. M. Chio, S. W. Shin, Comparision of immunomodulatory effects of water-extracted Adenophorae radix, Lriopis tuber, Dendroboii herba, Polygonati odorati rhizoma. Korean J. oriental physiology & pathology 21, 414 (2007).
  8. E. G. Han., S. Y. Cho, Effects of Codonopsis lanceolata water extract on the activities of antioxidative enzymes in carbon tetrachloride treated rats. J. Korean soc. Food Sci. Nutr. 26, 1181(1997).
  9. E. G. Han, I. S. Sung, H. G. Moon, S .Y. Cho, Effects of Codonopsis lancelata water extract on the level of lipid in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 27:940 (1998).
  10. N. Y. Kim., H. S. Chae, I. S. Lee, D. S. Kim, K. T. Seo, S. J. Park, Analysis of chemical composition and antioxidant activity of Codonopsis lanceolata Skin. J. Korean Soc. Food Sci. Nutri. 39, 1627 (2010). https://doi.org/10.3746/jkfn.2010.39.11.1627
  11. O. K. Kim, Antidiabetic and antioxidative effects of Lylii fructus in streptozotocin induced diabetic rats. J. Oil Chemist's soc. 25, 73(2008).
  12. J. R, Williamson, K. Chang, M. Franges, K. S, Hasan, Perspectives in diabetic hyperglycemic pseudohypoxia and diabetic complications. Diabetes, 42, 801 (1993).
  13. N. M. G, O,mera, R. bevery, O. collins, P. B, Johnson, A.H, Tomkin, G.H, Choi, Metabolism in alloxan-induced diabetic rabbits. Diabetes, 39, 626 (1990). https://doi.org/10.2337/diab.39.5.626
  14. S. Y. Cho, J. Y. Park, E. M, Park, M. S, Choi, M. K. Lee, S. M. Jeon, M. K, Jang, M. J, Kim, Y. B, Park, Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract. Clin. Chim. Acta., 317, 109 (2002). https://doi.org/10.1016/S0009-8981(01)00762-8
  15. R. B.Goldberg, Lipid disorders in diabetes. Diabetes Care,, 4, 561 (1981). https://doi.org/10.2337/diacare.4.5.561
  16. K. M. West, M. M. S, Ahuja, P. H. Bennett, The role of circulating glucose and triglyceride concentration and their interaction with other "risk factor" as determinants of asterial disease in nine diabetic population samples from WHO multinational study. Diabetes Care, 6, 361 (1983). https://doi.org/10.2337/diacare.6.4.361
  17. M. D. Meglasson, P. T. Burch, D. K. Berner, H. Najafi, F. M, Matschinsky, Identification of glucokinase as an alloxan-sensitive glucose sensor of the pancreatic-cell. Diabetes 35, 1163 (1986). https://doi.org/10.2337/diab.35.10.1163
  18. R. Ghosh, B. Mukherjee, M. A, Chatterjee, A novel effect of selenium on streptozotocin induced diabetic mice. Diabetes Res., 25, 165 (1994).
  19. S. Himeno, A. Takekawa, N. Imura, Special difference in hydroperoxide scavenging enzyme with special reference to glutathione peroxidase in guinea-pigs. Com p. Bodchem. Physiol. B., 104, 27 (1993). https://doi.org/10.1016/0305-0491(93)90334-2

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