Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지
DOI QR코드

DOI QR Code

Reduction of TNFα expression by Chungkookjang extracts in human breast cancer MDA-MB-231 cells

인간유방암 MDA-MB-231 세포에서 청국장추출물에 의한 TNFα 발현억제

  • Park, Jameon (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University) ;
  • Kang, Choong Kyung (Department of Nanobiotronics, Hoseo University) ;
  • Kim, Han Bok (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University)
  • 박잠언 (호서대학교 생명공학과 기초과학연구소) ;
  • 강충경 (호서대학교 나노바이오트로닉스학과) ;
  • 김한복 (호서대학교 생명공학과 기초과학연구소)
  • Received : 2016.07.04
  • Accepted : 2016.09.08
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Chungkookjang, fermented soybeans, contains diverse peptides produced during fermentation. Fermented soybean extracts containing the peptides can affect cellular signal transduction. Proliferation of human breast cancer MDA-MB-231 cells were repressed dependent on concentrations of fermented soybean extracts. Since fermented soybean extracts inhibited breast cancer cell's growth, and inflammation is related to cancer, it is determined whether it can suppress inflammatory $TNF{\alpha}$ expression. $TNF{\alpha}$ expression in MDA-MB 231 cells treated with fermented soybean extracts was repressed by that extracts. $TNF{\alpha}$ inhibitors were developed as drugs for autoimmune diseases. Since fermented soybean extracts suppressed $TNF{\alpha}$ expression, it can be developed as those drugs.

청국장은 대두발효식품으로 발효 중 생성된 다양한 펩타이드가 들어 있다. 이들 펩타이드가 포함된 청국장추출물을 세포에 처리하면 세포 신호전달에 영향을 미친다. 인간 유방암 MDA-MB-231 세포에 청국장추출물을 처리해 주었을 때 세포의 성장은 농도의존적으로 억제되었다. 청국장추출물이 유방암세포의 증식을 억제하였고 암과 염증이 관련이 있으므로, 청국장추출물이 염증 유전자의 하나인 $TNF{\alpha}$ 발현의 억제 여부를 알아 보았다. 인간유방암 MDA-MB 231 세포에 청국장 추출물을 처리하면 $TNF{\alpha}$ 발현은 억제되었다. $TNF{\alpha}$ 저해제는 자가면역질환 치료제로 개발되어 있다. 본 청국장추출물도 $TNF{\alpha}$ 억제 효과가 있으므로, 이들 자가면역 질환의 치료제로 개발될 수 있을 것이다.

Keywords

References

  1. Hwang, J.S., Sung, D.I., Lee, W.M., Chung, Y.S., and Kim, H.B. 2014. Ethanol extracts of Chungkookjang stimulate the proliferation and migration of human umbilical vascular endothelial cells. Korean J. Microbiol. 50, 223-226. https://doi.org/10.7845/kjm.2014.4037
  2. Hwang, J.S., Yoo, H.J., Song, H.J., Kim, K.K., Chun, Y.J., Matsui, T., and Kim, H.B. 2011. Inflammation-related signaling pathways implicating $TGF{\beta}$ are revealed in the expression profiling of MCF7 cell treated with fermented soybean, Chungkookjang. Nutr. Cancer 63, 645-652. https://doi.org/10.1080/01635581.2011.551987
  3. Kim, H.B., Lee, H.S., Kim, S.J., Yoo, H.J., Hwang, J.S., Chen, G., and Youn, H.J. 2007. Ethanol extract of fermented soybean, Chungkookjang, inhibits the apoptosis of mouse spleen, and thymus cells. J. Microbiol. 45, 256-261.
  4. Landskron, G., De la Fuente, M., Thuwajit, P., Thuwajit, C., and Hermoso, M.A. 2014. Chronic inflammation and cytokines in the tumor microenvironment. J. Immunol. Res. 2014, 149185.
  5. Lee, J.J., Lee, D.S., and Kim, H.B. 1999 Fermentation patterns of Chungkookjang and Kanjang by Bacillus licheniformis B1. Korean J. Microbiol. 35, 296-301.
  6. Lee, W.H., Wu, H.M., Lee, C.G., Sung, D.I., Song, H.J., Matsui, T., Kim, H.B., and Kim, S.G. 2014. Specific oligopeptides in fermented soybean extract inhibit NF-${\kappa}B$-dependent iNOS and cytokine induction by toll like receptor ligands. J. Med. Food 17, 1239-1246. https://doi.org/10.1089/jmf.2013.3070
  7. Ma, X. and Xu, S. 2013. TNF inhibitor therapy for rheumatoid arthritis. Biomed. Rep. 1, 177-184. https://doi.org/10.3892/br.2012.42
  8. Matsui, T., Yoo, H.J., Hwang, J.S., Lee, D.S., and Kim, H.B. 2004. Isolation of angiotensin-1-converting enzyme inhibitory peptide from Chungkookjang. Korean J. Microbiol. 40, 355-358.
  9. Sawada, M., Suzumura, A., and Marunouchi, T. 1992. $TNF{\alpha}$ induces IL-6 production by astrocytes but not by microglia. Brain Res. 583, 296-299. https://doi.org/10.1016/S0006-8993(10)80037-X
  10. Tanabe, K., Matsushima-Nishiwaki, R., Yamaguchi, S., Iida, H., Dohi, S., and Kozawa, O. 2010. Mechanisms of tumor necrosis factor-ɑ-induced interleukin-6 synthesis in glioma cells. J. Neuroinflammation 7, 16. https://doi.org/10.1186/1742-2094-7-16
  11. van Dullemen, H.M., van Deventer, S.J., Homes, D.W., Bijl, H.A., Jansen, J., Tytgat, G.N., and Woody, J. 1995. Treatment of Crohn's disease with anti-tumer neucrosis factor chimeric monoclonal antibody (cA2). Gastroenterol. 109, 129-135. https://doi.org/10.1016/0016-5085(95)90277-5
  12. Yoo, H.J., Hwang, J.S., and Kim, H.B. 2007. Mass analysis of isoflavones in Chungkookjang. Korean J. Microbiol. 43, 54-58.