Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Development of high-performance liquid chromatography methods for the anticoccidials: toltrazuril and diclazuril

고속액체크로마토그래피를 이용한 항원충제 분석법 개발: 톨트라주릴 및 디클라주릴

  • Jeong, Kyung Hun (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency) ;
  • Jeong, Miyoung (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency) ;
  • Park, Hae-Chul (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency) ;
  • Hossain, Md Akil (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency) ;
  • Kim, Daegyun (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency) ;
  • Lee, Kwang-Jick (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency) ;
  • Kang, Jeong Woo (Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency)
  • 정경훈 (농림축산검역본부 동물약품평가과) ;
  • 정미영 (농림축산검역본부 동물약품평가과) ;
  • 박해철 (농림축산검역본부 동물약품평가과) ;
  • 아킬후세인 (농림축산검역본부 동물약품평가과) ;
  • 김대균 (농림축산검역본부 동물약품평가과) ;
  • 이광직 (농림축산검역본부 동물약품평가과) ;
  • 강정우 (농림축산검역본부 동물약품평가과)
  • Received : 2017.07.28
  • Accepted : 2017.10.10
  • Published : 2017.12.31
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Abstract

This study was undertaken to develop new analytical methods for assessment of anticoccidials. High-performance liquid chromatography (HPLC) was found to be a fast, reliable, and practical method. The anticoccidials used in this experiment were toltrazuril and diclazuril, and the analysis factors were specificity, linearity, accuracy, repeatability, and intermediate precision. The linearity of each anticoccidial was better than 0.99, and the accuracies were 99.5% and 99.1% with relative SD of 0.5 and 0.4, respectively. To assess whether the developed HPLC method could be effectively applied, toltrazuril and diclazuril post-market veterinary products (five products) that are currently sold were tested. The results revealed no non-compliant items and the method was applied successfully. Therefore, the newly developed HPLC method for anticoccidial assessment described in this study may be useful as a reference method in the Korean Standards of Veterinary Pharmaceuticals for the analysis of toltrazuril and diclazuril.

Keywords

References

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