Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Decolorization of dyes by a purified laccase from Coprinus comatus

정제된 먹물버섯(Coprinus comatus) laccase에 의한 염료 탈색

  • Kim, Su Yeon (Department of Biochemistry, Kangwon National University) ;
  • Choi, Ji Young (Department of Biochemistry, Kangwon National University) ;
  • Choi, Hyoung T. (Department of Biochemistry, Kangwon National University)
  • 김수연 (강원대학교 자연과학대학 생화학과) ;
  • 최지영 (강원대학교 자연과학대학 생화학과) ;
  • 최형태 (강원대학교 자연과학대학 생화학과)
  • Received : 2017.04.04
  • Accepted : 2017.05.17
  • Published : 2017.06.30
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Abstract

An inky cap, Coprinus comatus synthesizes and secretes a laccase in the liquid yeast extract peptone dextrose medium. We have successfully purified the enzyme through the ion-exchange chromatography and the preparative gel electrophoresis. The estimated molecular weight was 67 kDa by the SDS-PAGE analysis. Optimum pH was pH 4.3 and optimum temperature was $25^{\circ}C$. The Km value was 0.45 mM and the Vmax was 0.0132 OD/min/unit for o-tolidine. Purified laccase showed 49.3% decolorizing activity against remazol brilliant blue R (RBBR) and 41.6% decolorizing activity against Poly R-478 after 12 h incubation.

먹물버섯 Coprinus comatus를 yeast extract peptone dextrose 액체배지에서 배양하고 laccase의 생성 분비를 확인하였다. 이 때 분비된 laccase를 이온교환수지 방법과 전기영동 분획수집방법으로 순수하게 정제하였다. 정제된 효소단백질의 분자량은 SDS-PAGE로 분석하였을 때 약 67 kDa으로 나타났고 최적산도는 pH 4.3, 최적온도는 $25^{\circ}C$로 확인되었다. 기질 o-tolidine에 대한 Km 값은 0.45 mM, Vmax 값은 0.0132 OD/min/unit로 나타났다. 정제된 laccase를 사용하여 염료 RBBR과 Poly R-478의 탈색을 분석한 결과 배양 12시간 후에 RBBR은 49.3% 탈색되었고 poly R-478은 41.6% 탈색되었다.

Keywords

References

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