The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparative Evaluation of Korean Medicine well-matched with Chicken through an inhibition of Pancreatic Lipase

닭고기와 잘 어울리는 췌장라이페이즈 억제능을 가진 한약재의 비교평가

  • Shin, Mi-Rae (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Lee, Young Cheol (Department of Herbology, College of Korean Medicine, Sangji University) ;
  • Seo, Bu-Il (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 신미래 (상지대학교 한의과대학 약리학교실) ;
  • 안효진 (상지대학교 한의과대학 약리학교실) ;
  • 이영철 (상지대학교 한의과대학 본초학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.06.13
  • Accepted : 2017.07.15
  • Published : 2017.07.30
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Abstract

Objectives : In this study, the inhibitory activities of Korean Medicine against pancreatic lipase in vitro and biochemical analyses in vivo were measured to determine its possibility as a well-matched sauce material with chicken. Methods : The inhibitory activity on pancreatic lipase enzyme of 11 samples were evaluated in vitro and then 5 samples were selected. The activity of pancreatic lipase was investigated using orlistat as a positive control. Animals were divided into eight groups (n=7). The experimental groups except for normal group were fed 60% high-fat diet for 7 days. 5 samples were orally administered at a dose of 200 mg/kg body weight and orlistat were orally administrated at a dose of 60 mg/kg body weight for 7 days. Biochemical anaylses of 5 samples were executed based on lipid parameters analysis. Results : Korean Medicines with an $IC_{50}$ of below 1 mg/kg were Scutellariae Radix, Gardeniae Fructus, Theae Folium Coptidis Radix, and Mori Cortex Radicis. Body weight change of Mori Cortex Radicis reduced significantly, however fecal triglyceride couldn't regulate effectively. The most excellent inhibitory effect of pancreatic lipase showed in Scutellariae Radix treatment and also regulated significantly serum triglyceride and total cholesterol. Moreover, the supplementation of Coptidis Radix excreted meaningfully triglyceride to fece. Conclusions : In conclusion, Coptidis Radix may exert anti-obesity effect by directly inhibiting pancreatic lipase, which would prevent the absorption of lipid from the small intestine. Besides, Mori Cortex Radicis may led to the decrease of the body weight via the different pathway.

Keywords

References

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