Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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A convergence study of antibacterial effect of solanum nigrum extract on candida albicans

까마중 추출물의 Candida albicans에 대한 항균효과에 대한 융합적 연구

  • Choi, Yu-Ri (Department of Dental Hygiene, Hallym Polytechnic University) ;
  • Kang, Min-Kyung (Department of Dental Hygiene, Hanseo University)
  • Received : 2018.08.28
  • Accepted : 2018.12.20
  • Published : 2018.12.28
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Abstract

The purpose of this study was to antibacterial effect of solanum nigrum extra in candida albicans. In this study, the antibacterial effects of 70% methanol extract and solanum nigraum were investigated. The content of flavonoids and polyphenol contents in solanum nigrum extraction. The antimicrobial activity was determined by spectrophotometric growth inhibition assay(optical density) and colony forming untis (CFU) for the candida albicans. Total contents were flavonoids and polyphenols of solanum nigrum, the result was $8.23{\pm}0.13mg/g$ and $14.97{\pm}1.58mg/g$, respectively. The CFU results showed lower CFU counts in the experimental groups compared to the control group. And the result of the bacterial viability was decreased in the experimental group. Therefore the extracts of solanum nigrum were found to have antimicrobial activity in candida albicans. From the these results, solanum nigrum extracts have a possibility to be a candidate for natural antibacterial components in dental products.

본 연구의 목적은 까마중추출물의 캔디다 알비칸스의 항균력을 확인하고자 하였다. 연구를 위해 까마중을 70퍼센트 메탄올에 추출하였다. 추출물의 성분분석을 위해 까마중 추출물의 플라보노이드와 폴리페놀의 함량을 확인하였다. 까마중 추출물의 캔디다 알비칸스의 항균력을 확인하기 위하여 생존률을 OD값으로 측정하였고, 증식능력을 CFU로 측정하였다. 까마중 추출물의 플라보노이드와 폴리페놀의 함량을 분석한 결과 각 각 $8.23{\pm}0.13mg/g$와 $14.97{\pm}1.58mg/g$로 나타났다. CFU 결과, 대조군과 비교하여 실험군에서 CFU 값이 낮게 나타났다. 균의 활성도를 평가한 결과, 대조군과 비교하여 실험군에서 더 낮은 균활성도 값을 보여 칸디다 알비칸스에 대한 까마중 추출물의 항균력을 확인할 수 있었다. 이러한 연구 결과, 까마중 추출물은 칸디다균뿐만 아니라 치과분야에서 천연 항균제로 사용될 수 있을 가능성이 보인다.

Keywords

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Fig. 1. Result of Colony forming unit (CFU) (a):0 mg/mL(Control), (b):5 mg/mL, (c):10 mg/mL, (d):20 mg/mL, (e):40 mg/mL.

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Fig. 2. Result of Bacteria viability(%) Same alphabet letters was not statistic significant different Different alphabet letters was not statistic significant different

Table 1. The flavonoid and polyphenol compound contents in the extracts of solanum nigrum

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