Figure 1. Representative 1H-NMR spectrum of aqueous extracts of zebrafish. High and low field spectra were magnified. Ala, Gln and Glu indicate alanine, glutamine and glutamate, respectively.
Figure 2. Data normalization plot (control-based z-score).
Figure 2. PLS-DA score plot of zebrafish exposed to BIT (●, control; ●, exposure).
Figure 4. VIP plot. The VIP plot displays the top 15 most important metabolite features identified by PLS-DA. (Metabolites of VIP > 1).
Figure 5. ROC curve. The ROC curves are the comparison of control and BIT exposed group. AUC values: Dimethylamine (0.96) and N-Acetylaspartate (0.92).