Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Optimization of ginseng hairy roots culture and its ginsenoside analysis

  • Ji, Joong Gu (Department of Oriental Health Care, Joongbu University) ;
  • Yoo, Sun Kyun (Department of Food and Biotechnology, Joongbu University)
  • Received : 2018.11.08
  • Accepted : 2018.12.12
  • Published : 2018.12.31
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Abstract

Hairy root culture of ginseng is industrially prospected because the cultivation period of ginseng is relatively long. In this study, the effect of medium concentration and sucrose concentration on hairy root culture of ginseng was evaluated. The optimization of ginseng hairy roots transformed by Agrobacterium rhizogene were performed liquid medium. The MS(Murashinge & Skoog basal medium) concentration was selected with 1/2 strength MS and the optimal sucrose concentration was determined at 2-3%(w/v). At the optimum culture condition, The yield (the ratio of weight of grown hairy root cultures to weight of fresh ginseng hairy roots) and production rate of ginseng root were 19.42 times and 5.73 g/l-day. The major ginsenosides were Rb group, Re and Rg1. The produced total ginsenoside content in the solid medium was 9.87 (mg/g) and increased 1.34 times in the liquid medium (13.23 mg/g). In solid culture, the contents of ginsenosides Rb, Re and Rg1 were 2.14, 3.65 and 1.87 mg/g, respectively. In liquid culture, the contents of ginsenosides Rb, Re and Rg1 were 3.54, 4.12 and 2.63 mg/g, respectively.

Keywords

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Fig. 1. Ginseng hairy root cultures (A; solid culture and B; liquid culture) transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃, pH 5.8, 3%(w/v) sucrose, and 1/2 strength of MS medium for 5 weeks.

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Fig. 2. Ginseng hairy root cultures transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃, pH 5.8, 2%(w/v) sucrose for 5 weeks. MS concentrations were 1/4, 1/2, 1, 2, and 3 strength of MS.

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Fig. 3. Ginseng hairy root cultures transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃, pH 5.8, 1/2 strength of MS for 5 weeks. The sucrose concentrations were 1, 2, 3, 5, and 10% (w/v).

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Fig. 4. The TLC chromatogram and analysis of ginsenoside produced from root culture. HRP and HRF represent the ginseng hairy root cultures grown at Petri-dish solid and flask liquid culture.

Table 1. Yield and productivity of ginseng hairy root cultures transformed by Agrobacterium rhizogene. Hairy roots grew at 25℃ and pH 5.8 for 5 weeks. MS concentrations were 1/4, 1/2, 1, 2, and 3 strengrt of MS. The sucrose concentrations were 1, 2, 3, 5, and 10% (w/v)

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Table 2. The amount of ginsenosides of ginseng hairy root cultures transformed by Agrobacterium rhizogene at the optimized conditions for 5 weeks

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