Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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The Study of Cosmeceutical Activities from Lentinula edodes extracts and Application a Natural Cosmetic Material

표고버섯 추출물의 화장품약리활성 검증과 천연화장품 소재로써의 활용에 관한 연구

  • Seo, Myeong-Seong (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Jang, Young-Ah (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University)
  • 서명성 (대구한의대학교 바이오융합학부) ;
  • 장영아 (대구한의대학교 바이오융합학부) ;
  • 이진태 (대구한의대학교 바이오융합학부)
  • Received : 2018.09.17
  • Accepted : 2018.12.12
  • Published : 2018.12.31
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Abstract

This study is for checking the possibility of Lentinula edodes as cosmetic materials. For this we carried out biological active evaluation about anti-oxidant and anti-inflammatory effects by Lentinula edodes extracts. We extracted Lentinula edodes with water and 70% ethanol and then in order to evaluate anti-oxidant activity we treated samples by concentrations (100, 500, 1000) ${\mu}g/ml$ and carried out 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, the activity of 2,2'-azino-bis ( 3-ethylbenzothiazoline-6-sulphonic acid )-diammonium salt (ABTS) cation radical scavenging and superoxide dismutase(SOD) like activity. Also, in order to evaluate effect of anti-inflammatory the samples in macrophages(RAW 264.7 cells), we carried out evaluation of cell viability, nitric oxide inhibitory activity western blot. The results of DPPH, $ABTS^+$ radical scavenging activity and SOD-like activity of the Lentinula edodes extracts increased in dose-dependent manner. The cytotoxic of samples by MTT assay showed no toxicity at the concentrations of 10, 25 and $50{\mu}g/ml$ of Lentinula edodes extract. Nitric oxide inhibition activity results showed that the extracts reduced NO productions in a concentration-dependent manner. Expression of inflammatory cytokines as $TNF-{\alpha}$, $PGE_2$ and $IL-1{\beta}$ decreased in a concentration-dependent manner and iNOS and COX-2 proteins expression rates were decreased significantly in western blot analysis. From the results of the experiment, it was comfirmed that the Lentinula edodes extracts had excellent anti-oxidant and anti-inflammatory effect and could be used as a safe natural cosmetic material in the future.

본 연구는 화장품 소재로써 표고버섯의 기능성을 확인하기 위한 것이다. 이를 위해 우리는 표고버섯 추출물을 사용하여 항산화, 항염증 효과에 대한 생물학적 활성 평가를 수행하였다. 표고버섯을 열수와 70% 에탄올로 추출한 후 농도 (100, 500, 1000) ${\mu}g/ml$ 에 따라 처리하여 항산화 실험인 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능, 2,2'-azino-bis ( 3-ethylbenzothiazoline - 6-sulphonic acid )-diammonium salt (ABTS) 양이온 라디칼 소거능과 Superoxide dismutase (SOD) 유사활성을 평가하였다. 또한 항염증 효과를 평가하기 위해 대식세포(Raw 264.7)를 이용해 샘플의 독성평가와 nitric oxide 생성 저해 활성을 평가하였다. 표고버섯 추출물의 DPPH 라디칼 소거능, $ABTS^+$ 라디칼 소거능과 SOD유사활성 측정결과 $1,000{\mu}g/ml$ 농도에서 농도 의존적으로 활성이 증가하였다. MTT assay를 통한 샘플의 독성평가 결과로는 표고버섯 추출물의 10, 25, $50{\mu}g/ml$의 농도에서 독성이 나타나지 않았다. Nitric oxide 저해 활성 결과에 따르면 표고버섯 추출물의 농도 의존적으로 NO가 저해됨을 보아 항염증 효과가 우수함을 알 수 있다. 표고버섯은 염증성 Cytokine인 $TNF-{\alpha}$, $PGE_2$, $IL-1{\beta}$의 활성을 낮추었으며 Western blot 실험결과 iNOS와 COX-2 단백질의 발현을 최고농도인 $25{\mu}g/ml$ 에서 유의적으로 억제하는 것을 확인하였다. 상기 실험결과로부터 표고버섯의 우수한 항산화, 항염증 효능을 확인하였으며 향후 안전한 천연 화장품 소재로 사용될 수 있음을 보여준다.

Keywords

HGOHBI_2018_v35n4_1003_f0001.png 이미지

Fig. 1. DPPH radical scavenging activity(%) of dependent on concentration from extracts of Lentinula edodes.

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Fig. 2. ABTS+ cation radical scavenging activity(%) of dependent on concentration from extracts of Lentinula edodes. LEW : Lentinula edodes extracted with water.

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Fig. 3. Superoxide dismutase like activity(%) of dependent on concentration from extracts of Lentinula edodes.

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Fig. 4. Cell viability of dependent on concentration of Lentinula edodes extracts(LEW) using MTT assay.

HGOHBI_2018_v35n4_1003_f0005.png 이미지

Fig. 5. Inhibitory effects of extracts of Lentinula edodes extracts(LEW) on the production of nitric oxide Raw 264.7 cells.

HGOHBI_2018_v35n4_1003_f0006.png 이미지

Fig. 6. Inhibitory effects of extracts of Lentinula edodes extracts(LEW) on production of pro-inflammatory cytokines.

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Fig. 7. Effects of Lentinula edodes extracts (LEW) on LPS-induced iNOS, COX-2 protein expression.

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