대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제44권1호
  • /
  • Pages.9-14
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  • 2018
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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용담화 추출물의 미백 활성 연구

A Study on the Whitening Activities of Gentiana scabra Extract

  • 이현상 (매니아시아 알엔디센터) ;
  • 석지현 (매니아시아 알엔디센터) ;
  • ;
  • 김윤정 (매니아시아 알엔디센터)
  • 투고 : 2017.12.27
  • 심사 : 2018.03.06
  • 발행 : 2018.03.31
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초록

본 연구에서는 항산화 및 미백 소재의 개발을 위해 다양한 추출법을 이용하여 용담화 추출물을 제조하고 항산화 효과 및 멜라닌 생합성 저해능을 평가하였다. 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거활성을 평가한 결과 용담화 추출물의 항산화 활성은 모두 농도 의존적으로 증가하였다. 또한 용담화 추출물은 tyrosinase 활성을 저해시켰으며, B16F10 세포에서 멜라닌 생성을 감소시키는 효과를 보였다. 멜라닌 생합성에 관여하는 유전자의 발현에 미치는 영향을 알아보기 위해 tyrosinase-related protein (TRP)-1 및 2 mRNA 발현을 확인한 결과 용담화 추출물에 의해 TRP-1 및 2의 발현이 감소되는 것을 확인할 수 있었다. 이러한 결과로 보아 용담화 추출물은 항산화 및 미백 기능성 화장품 소재로서의 응용이 가능할 것으로 사료된다.

In order to find new functional materials for the cosmetics application, we investigated the anti-oxidant and anti-melanogenic properties of Gentiana scabra extracts (GSE), which were prepared by the various extraction methods. Results showed that GSE had high DPPH radical scavenging activity in a dose-dependent manner. Also, GSE inhibited the prodution of melanin in B16F10 melanoma cell as well as tyrosinase activity. We also found that GSE inhibited mRNA expression of tyrosinase-related protein (TRP)-1 and 2. In conclusion we suggest that GSE is applicable to cosmetics as a potential ingredient for their anti-oxidant and whitening effects.

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