Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Ethanol Extracts from Astilbe chinensis (Maxim.) Franch. Et Savat. Exhibit Inhibitory Activities on Oxidative Stress Generation and Viability of Human Colorectal Cancer Cells

노루오줌 에탄올 추출물의 산화스트레스 및 대장암 세포 억제활성

  • Nho, Jong Hyun (National Development Institute of Korean Medicine) ;
  • Jang, Ji Hun (National Development Institute of Korean Medicine) ;
  • Jung, Ho Kyung (National Development Institute of Korean Medicine) ;
  • Lee, Mu Jin (National Development Institute of Korean Medicine) ;
  • Sim, Mi Ok (National Development Institute of Korean Medicine) ;
  • Jeong, Da Eun (National Development Institute of Korean Medicine) ;
  • Cho, Hyun Woo (National Development Institute of Korean Medicine)
  • Received : 2018.01.31
  • Accepted : 2018.04.09
  • Published : 2018.04.30
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Abstract

Background: Astilbe chinensis (Maxim.) Franch. Et Savat. is a plant belonging to Saxifragaceae family and contains various active ingredients including astilbin and bergenin. It has been used as a traditional Korean medicine to improve fever, pain, and cough. Recently, a number of Korean medical resources have been studied for cancer and inflammation treatment, but A. chinensis (Maxim.) Franch. Et Savat. has not yet been investigated. Consequently, this study investigated the inhibitory effect of ethanol extracts from A. chinensis (Maxim.) Franch. Et Savat. (ARE) on oxidative stress and colorectal cancer using RAW264.7 and the human colorectal cancer cell line HCT-116. Methods and Results: In total, $500{\mu}g/m{\ell}$ ARE reduced cell viability by $38.96{\pm}1.32%$, and increased caspase-3 activity by $133.08{\pm}3.41%$ in HCT-116 cells. Moreover, TUNEL signaling and the early apoptosis ratio ($34.56{\pm}1.67%$) increased by $500{\mu}g/m{\ell}$ ARE treatment. $H_2O_2$-induced oxidative stress and cell death were diminished by $500{\mu}g/m{\ell}$ ARE treatment through decreasing ROS (reactive oxygen species). Conclusions: The inhibitory effects of ARE against human colorectal cancer cells is mediated by apoptosis and caspase-3 activation, and $H_2O_2$-induced ROS generation and cell death are decreased by ARE treatment in RAW264.7 cells. However, further study is required to explore how ARE treatment is involved in the signaling pathway to decrease ROS.

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References

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