Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Extraction of Low Molecular Weight Ginsenosides from Adventitious Roots Culture of Wild Mountain Ginseng by Steam Processing

증숙 처리에 의한 산삼 부정 배양근의 저분자 진세노사이드 추출

  • Lee, Ye Ji (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Kim, Hee Kyu (Gangwon Forest Science Institute) ;
  • Go, Eun Ji (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Choi, Jae Hoo (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Jo, Ah Reum (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Kim, Chul Joong (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Choi, Seon Kang (Department of Agricultural Life Sciences, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University)
  • 이예지 ((주)화진바이오코스메틱) ;
  • 김희규 (강원도산림과학연구원) ;
  • 고은지 (강원대학교 생약자원개발학과) ;
  • 최재후 (강원대학교 생물자원과학과) ;
  • 조아름 ((주)화진바이오코스메틱) ;
  • 김철중 ((주)화진바이오코스메틱) ;
  • 이재근 ((주)화진바이오코스메틱) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 최선강 (강원대학교 농생명산업학과) ;
  • 유창연 (강원대학교 생물자원과학과)
  • Received : 2018.02.28
  • Accepted : 2018.04.23
  • Published : 2018.04.30
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Abstract

Background: Hot steaming is known to be effective in improving the biological activities of plant extracts by breaking down useful compounds to low molecular weight ones. Methods and Results: This study aimed to develop an optimal extraction and steam processing method for enhancing the low molecular ginsenoside contents of the adventitious roots culture of wild mountain ginseng. The total ginsenoside was optimally extracted when 70% EtOH was used at $50^{\circ}C$, whereas low molecule ginsenoside such as Rg2, Rh1, Rh4 and Rk1 could be extracted using 70% EtOH at $70^{\circ}C$. The adventitious roots culture of wild mountain ginseng is known to contain four major ginsenosides, i.e., Rb2, Rb1, Rg1 and Rd, however new ginsenosides Rg6, Rh4, Rg3, Rk1 and Rg5 were new abundantly obtaind after steam processing method was applied. The contents of total ginsenosides were the highest when thermal steam processing was conducted at $120^{\circ}C$ for 120 min. Unlike ginsenosides such as Rg1, Re, Rb1, Rc, Rb2, and Rh1, which decreased after steam processing, Rg3, Rk1, and Rg5 increased after thermal processing. Steam processing significanltly reduced the content of Rb1, increased that of Rg6 by about ten times than that in the adventitious roots culture of wild mountain ginseng. Conclusions: Our study showed that the optimal extraction and steam processing method increased the content of total ginsenosides and allowed the extraction of minor ginsenosides from major ones.

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References

  1. Ahn S, Siddiqi MH, Aceituno VC, Simu SY, Zhang J, Perez ZEJ, Kim YJ and Yang DC. (2016). Ginsenoside Rg5: Rk1 attenuates TNF-$\alpha$/IFN-$\gamma$-induced production of thymus-and activation-regulated chemokine(TARC/CCL17) and LPS-induced NO production via downregulation of NF-${\kappa}B$/p38 MAPK/ STAT1 signaling in human keratinocytes and macrophages. In Vitro Cellular and Developmental Biology-Animal. 52:287-295. https://doi.org/10.1007/s11626-015-9983-y
  2. Choi JE, Nam KY, Li X, Kim BY, Cho HS and Hwang KB. (2010). Changes of chemical compositions and ginsenoside contents of different root parts of ginsengs with processing method. Korean Journal of Medicinal Crop Science. 18:118-125.
  3. Corbit RM, Ferreira JFS, Ebbs SD and Murphy LL. (2005). Simplified extraction of ginsenosides from American ginseng(Panax quinquefolius L.) for high-performance liquid chromatographyultraviolet analysis. Journal of Agricultural and Food Chemistry. 53:9867-9873. https://doi.org/10.1021/jf051504p
  4. Ha YJ, Kim MR and Yoo SK. (2015). Process optimization of ginseng berry extract using mixed solvent and its ginsenoside analysis. Journal of the Korea Academia-Industrial Cooperation Society. 16:7794-7800. https://doi.org/10.5762/KAIS.2015.16.11.7794
  5. Hong HD, Kim YC, Rho JH, Kim KT and Lee YC. (2007). Changes on physicochemical properties of Panax ginseng C. A. Meyer during repeated steaming process. Journal of Ginseng Research. 31:222-229. https://doi.org/10.5142/JGR.2007.31.4.222
  6. Hong MH, Lim HK, Park JE, Jun NJ, Lee YJ, Cho MJ and Kim SM. (2008). The antihypertensive and vasodilating effects of adventitious root extracts of wild ginseng. Journal of the Korean Society for Applied Biological Chemistry. 51:102-107. https://doi.org/10.3839/jabc.2008.017
  7. Jovanovski E, Bateman EA, Bhardwaj J, Fairgrieve C, Mucalo I, Jenkins AL and Vuksan V. (2014). Effect of Rg3-enriched Korean red ginseng(Panax ginseng) on arterial stiffness and blood pressure in healthy individuals: A randomized controlled trial. Journal of the American Society of Hypertension. 8:537-541. https://doi.org/10.1016/j.jash.2014.04.004
  8. Kang KS, Kim HY, Pyo JS and Yokozawa T. (2006). Increase in the free radical scavenging activity of ginseng by heatprocessing. Biological and Pharmaceutical Bulletin. 29:750-754. https://doi.org/10.1248/bpb.29.750
  9. Kim CJ, Seong ES, Yoo JH, Lee JG, Kim NJ, Choi SK, Lim JD and Yu CY. (2016). Biological activity of Panax ginseng C. A. Meyer culture roots fermented with microorganisms. Korean Journal of Medicinal Crop Science. 24:191-197. https://doi.org/10.7783/KJMCS.2016.24.3.191
  10. Kim CS, Jang DS and Che SY. (2006). Histological characteristics of Korean red ginseng in steaming processes. Korean Journal of Medicinal Crop Science. 14:36-40.
  11. Kim HG, Kim KY and Cha CJ. (2007). Screening for ginsengfermenting microorganisms capable of biotransforming ginsenosides. Korean Journal of Microbiology. 43:142-146.
  12. Kim JH and Kim JK. (2005). Effect of extracting conditions on chemical compositions of Korean mountain ginseng extract. Journal of Korean Society of Food Science and Nutrition. 34:862-868. https://doi.org/10.3746/jkfn.2005.34.6.862
  13. Kim JK, Kim SH, Lee DS, Lee DJ, Kim SH, Chung SK and Yang HO. (2013). Effects of fermented ginseng on memory impairment and ${\beta}$-amyloid reduction in Alzheimer's disease experimental models. Journal of Ginseng Research. 37:100-107. https://doi.org/10.5142/jgr.2013.37.100
  14. Kim MY, Lee JB, Yang BW, Park JD and Ko SK. (2017). The comparison of ginseng saponin composition and contents in steaming ginseng radix. Yakhak Hoeji. 61:274-280. https://doi.org/10.17480/psk.2017.61.5.274
  15. Lee HU, Bae EA, Han MJ, Kim NJ and Kim DH. (2005). Hepatoprotective effect of ginsenoside Rb1 and compound K on tert-butyl hydroperoxide-induced liver injury. Liver International. 25:1069-1073. https://doi.org/10.1111/j.1478-3231.2005.01068.x
  16. Lee JH, Kwon KR and Cha BC. (2008). Component analysis of cultivated ginseng, red ginseng, cultivated wild ginseng, and red wild ginseng using HPLC method. Journal of Pharmacopuncture. 11:87-95.
  17. Lee JW, Mo EJ, Choi JE, Jo YH, Jang H, Jeong JY, Jin Q, Chung HN, Hwang BY and Lee MK. (2016). Effect of Korean red ginseng extraction conditions on antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content: Optimization using response surface methodology. Journal of Ginseng Research. 40:229-236. https://doi.org/10.1016/j.jgr.2015.08.001
  18. Lee NR, Han JS, Kim JS and Choi JE. (2011). Effects of extraction temperature and time on ginsenoside content and quality in ginseng(Panax ginseng) flower water extract. Korean Journal of Medicinal Crop Science. 19:271-275. https://doi.org/10.7783/KJMCS.2011.19.4.271
  19. Nam SH, Rhee YK, Hong HD, Lee YC, Kim YC, Shin KS and Cho CW. (2012). Immuno-modulatory activity of the crude polysaccharide from wild ginseng adventitious root. Korean Journal of Food and Nutrition. 25:755-761. https://doi.org/10.9799/ksfan.2012.25.4.755
  20. Park CK, Jeon BS and Yang JW. (2003). The chemical components of Korean ginseng. Food Industry and Nutrition. 8:10-23.
  21. Park EH, Kim YJ, Yamabe N, Park Sh, Kim HK, Jang HJ, Kim JH, Cheon GJ, Ham JY and Kang KS. (2013). Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed american ginseng on human gastric cancer cell. Journal of Ginseng Research. 38:22-27.
  22. Shin CS, Lee DH, Kim SH, Shin MH, Jeong CH and Shim KH. (2010). Ginsenoside contents and antioxidative activities from red ginseng treated with high hydrostatic pressure. Journal of Agriculture and Life Science. 44:133-140.
  23. Shin MH. (2001). Study of mountain ginseng adventitious culture and application. Journal of the Society of Cosmetic Scientists of Korea. 27:45-56.
  24. Siddiqi MH, Siddiqi MZ, Ahn SG, Kang SR, Kim YJ, Veerappen K, Yang DU and Yang DC. (2014). Stimulative effect of ginsenosides Rg5: Rk1 on murine osteoblastic MC3T3-E1 cells. Phytotherapy Research. 28:1447-1455. https://doi.org/10.1002/ptr.5146
  25. Song CH, Seo YC, Choi WY, Lee CG, Kim DU, Chung JY, Chung HC, Park DS, Ma CJ and Lee HY. (2012). Enhancement of antioxidative activity of Codonopsis lanceolata by stepwise steaming process. Korean Journal of Medicinal Crop Science. 20:238-244. https://doi.org/10.7783/KJMCS.2012.20.4.238
  26. Yang BW, Han ST and Ko SK. (2006). Quantitative analysis of ginsenosides in red ginseng extracted under various temperature and time. Korean Journal of Pharmacognosy. 37:217-220.
  27. Yi EJ, Lee JM, Yi TH, Cho SC, Park YJ and Kook MC. (2012). Biotransformation of ginsenoside by Lactobacillus brevis THK-D57 isolated from kimchi. Korean Journal of Food and Nutrition. 25:629-636. https://doi.org/10.9799/ksfan.2012.25.3.629