Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Improvement of the electro-transformed cell yield for Bifidobacterium sp. with large DNA

  • Park, Min Ju (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University) ;
  • Park, Myeong Soo (Research Center, BIFIDO Co., Ltd.) ;
  • Ji, Geun Eog (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University)
  • Received : 2019.01.08
  • Accepted : 2019.02.26
  • Published : 2019.04.30
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Abstract

In this study, the poor electro-transformant yield of large DNA in bifidobacteria was improved by increasing the DNA concentration, which was amplified by enhancing electroporation conditions: treating the cell wall weakening agent and cell membrane permeabilizing molecule as well as changing the electrical parameter. In the enhanced conditions, the electroporation frequency increased from 15 to 71 times compared to the initial conditions at the same DNA concentration. As the DNA concentration increased, the difference in the electroporation frequency between the two conditions became greater, and the curve of the enhanced conditions seemed to be saturated with a DNA concentration over $4{\mu}g$. The present study provided a clue to the recovery of the electroporation frequency with large DNA and formulated the relationship between the DNA concentration, the DNA size and the electroporation frequency in bifidobacteria. Therefore, this study will contribute to the expansion of molecular genetic studies of bifidobacteria.

Keywords

SPGHB5_2019_v51n2_188_f0001.png 이미지

Fig. 1. Investigation of electro-transformation frequency of large size DNA under initial conditions (closed circle) and enhanced conditions (open circle) across multiple plasmid DNA concentrations.

Table 1. Bacterial strains and plasmids used in this study

SPGHB5_2019_v51n2_188_t0001.png 이미지

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