Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Physiological activities of Pyrus pyrifolia Nakai, malt, Ziziphus jujuba Mill., Leonurus japonicus Houtt., Scutellaria baicalensis, and their mixtures

배, 맥아, 대추, 익모초, 황금 및 이들 혼합물의 생리활성

  • SooJung Lee (Department of Food Science and Nutrition, Gwangju University) ;
  • HyunJun Kim (Division of Administration, Herbbori Co.) ;
  • Mi Ja Chung (Department of Food Science and Nutrition, Gwangju University)
  • Received : 2023.11.12
  • Accepted : 2023.11.27
  • Published : 2023.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the anti-inflammatory, trypsin activity, and antioxidant effects of 11 kinds of plant extracts to discover materials for developing optimal mixtures that improve inflammation and help digestion. Ziziphus jujuba Mill. (ZJ), Leonurus japonicus Houtt. (LJ), Scutellaria baicalensis (SB), Platycodon grandiflorum, and Aster scaber extracts had excellent anti-inflammatory effects by reducing excessive nitric oxide (NO) and tumor necrosis factor-α content in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The malt (MA), Pyrus pyrifolia Nakai (PP), Raphanus sativus L., Platycodon grandiflorum extracts among the 11 kinds of plant extracts had high trypsin activity. The antioxidant activity of the plant extracts was examined by the DPPH radical scavenging activity, and the SB, PE, JU, and MA extracts had high antioxidant activity. Therefore, PP, MA, ZJ, LJ, and SB were selected to develop optimal mixtures that improve inflammation and help digestion. The extract of plant mixture containing PP, MA, ZJ, LJ, and SB in the ratio 1:1:2:1:2 (w/w) significantly inhibited NO production than the extract of PP, MA, ZJ, LJ, and SB, respectively, in LPS-stimulated RAW 264.7 macrophages. The DPPH radical scavenging activity of the mixture extract was significantly higher than the extract of PP, MA, ZJ, and LJ, respectively.

본 연구는 염증을 개선하고 소화에 도움을 주는 최적 혼합물 개발을 위한 소재 발굴을 위해 11종류 식물 추출물을 이용하여 항염증, 트립신 활성 및 항산화 효과를 알아보았다. LPS로 염증이 유도된 RAW 264.7 대식세포에서 대추(ZJ), 익모초(LJ), 황금(SB), 도라지 그리고 참취 추출물은 과분비 되는 NO와 TNF-α 함량을 감소시켜 뛰어난 항염증 효과가 있었다. 11개의 시료 중 맥아(MA), 배(PP), 무 그리고 도라지 추출물은 트립신 활성이 높았다. DPPH 라디칼 소거작용으로 항산화 효과를 알아본 결과 SB, PP, ZJ 그리고 MA 추출물이 높은 항산화 효과가 있었다. 따라서 염증을 개선하면서 소화에 도움을 주는 최적 혼합물 개발을 위해 배, 맥아, 대추, 익모초 그리고 황금을 선택하였다. LPS로 자극한 RAW 264.7 대식세포에서 PP, MA, ZJ, LJ 및 SB(1:1:2:1:2, w/w)을 함유하는 혼합물의 추출물은 각각의 PP, MA, ZJ, LJ 및 SB의 추출물보다 NO 생산을 현저하게 억제하였다. 혼합물 추출물의 DPPH 라디칼 소거작용은 PP, MA, ZJ 및 LJ의 각각의 추출물보다 현저하게 높았다.

Keywords

Acknowledgement

Following are results of a study on the "Leaders in INdustry-university Cooperation 3.0" Project, supported by the Ministry of Education and National Research Foundation of Korea.

References

  1. Bae HK, Seo BI. Inhibitory activities of Rehmanniae radix 30% ethanol extract on acute gastritis and peptic ulcers. Kor J Herbol, 34, 1-14 (2019)
  2. Cho EH, Choi AR, Choi SJ, Kim SY. Amylase activity of radish and stability in processing. J Korean Soc Food Sci Nutr, 38, 812-815 (2009)
  3. Choi YN, Choi YK, Nan L, Choo BK. Anti-oxidant and anti-inflammatory effects of ethanol extracts from Leonurus japonicus Houtt. on LPS-induced RAW 264.7 cells. Korean J Org Agric, 28, 659-677 (2020)
  4. Chung MJ, Lee S, Park YI, Lee J, Kwon KH. Neuroprotective effects of phytosterols and flavonoids from Cirsium setidens and Aster scaber in human brain neuroblastoma SK-N-SH cells. Life Sci, 148, 173-182 (2016) https://doi.org/10.1016/j.lfs.2016.02.035
  5. Chung MJ, Randey RP, Choi JW, Sohng JK, Choi DJ, Park YI. Inhibitory effects of kaempferol-3-O-rhamnoside on ovalbumin-induced lung inflammation in a mouse model of allergic asthma. Int Immunopharmacol, 25, 302-310 (2015) https://doi.org/10.1016/j.intimp.2015.01.031
  6. Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cell Signal, 13, 85-94 (2001) https://doi.org/10.1016/S0898-6568(00)00149-2
  7. Hong CE, Chun YH, Lyu SY. Anti-inflammatory and antioxidant effects of Zizyphus jujuba Miller var. inermis extracts. Kor J Pharmacogn, 50, 86-95 (2019)
  8. Jung MS, Lee GS, Chae HJ. In vitro biological activity assay of ethanol extract of turnip. J Korean Soc Appl Biol Chem, 47, 67-71 (2004)
  9. Kaplanski G, Marin V, Montero-Julian F, Mantovani A, Farnarier C. IL-6: A regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Inmmunol, 24, 25-29 (2003) https://doi.org/10.1016/S1471-4906(02)00013-3
  10. Kim EH, Gwak JY, Chung MJ. Immunomodulatory activity of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla extracts in macrophage cells. J Korean Soc Food Sci Nutr, 47, 1069-1075 (2018) https://doi.org/10.3746/jkfn.2018.47.11.1069
  11. Kim EH, Shim YY, Lee HI, Lee SH, Reaney MJT, Chung MJ. Astragalin and isoquercitrin isolated from Aster scaber suppress LPS-induced neuroinflammatory responses in microglia and mice. Foods, 11, 1505 (2022)
  12. Kim JY, Yang HS, Kang H, Choe JS, Hwang IG. Chemical composition, antioxidant and anti-inflammatory potential in whole, flesh, and peels of Codonopsis lanceolata roots. J Korean Soc Food Sci Nutr, 52, 26-39 (2023)
  13. Kim MJ, Kim YG, Kim HS, Cheong C, Jang KH, Kang SA. Effects of antioxidant activities in ethanol extract of apple, peel, grape peel, and sweet potato peel as natural antioxidant. JKAIS, 15, 3766-3773 (2014)
  14. Kim MK, Kim DY. Anti-inflammatory effect of barley leaf ethanol extract in LPS-stimulated RAW 264.7 macrophage. Korean J Food Preserv, 22, 735-743 (2015) https://doi.org/10.11002/kjfp.2015.22.5.735
  15. Kim NY. Effect of antioxidation and inhibition of melanogenesis from Scutellaria baicalensis extract. Kor J Aesthet Cosmetol, 12, 41-47 (2014)
  16. Kim SY, Oh JY, Kim EA, Heo SJ, Kim KN, Jeon YJ. Inhibition of lipid accumulation in 3T3-L1 adipocytes by different enzymatic hydrolysates of dried red sea cucumber Stichopus japonicus. Korean J Fish Aquat Sci, 53, 707-716 (2020)
  17. Lee G, Na GH, Kim WK, Baik MY, Lee HJ, Hwang JK. Anti-inflammatory effect of cultivated wild Panax ginseng extracts at various ages in RAW 264.7 macrophages. Food Eng Prog, 21, 201-207 (2017) https://doi.org/10.13050/foodengprog.2017.21.3.201
  18. Lee HI, Gwak JY, Chung MJ. Inhibitory effects of Platycodon grandiflorum, Codonopsis lanceolata, corn silk, Acanthopanax senticosus against nitric oxide-induced cytotoxicity in SK-N-SH cells. J Korean Soc Food Sci Nutr, 48, 823-832 (2019) https://doi.org/10.3746/jkfn.2019.48.8.823
  19. Lee J. A survey on intake of protein supplement of university students majoring in physical education. J Korean Soc Food Sci Nutr, 43, 1607-1613 (2014) https://doi.org/10.3746/jkfn.2014.43.10.1607
  20. Lee JI, Chung MY. Barley sprout extract and luteolin mitigate LPS-mediated inflammation in RAW 264.7 murine macrophages via histone acetyltrasferase activity inhibition. J Korean Soc Food Sci Nutr, 50, 1282-1291 (2021)
  21. Lee SM, Park JG, Lee YH, Lee CG, Min BS, Kim JH, Lee HK. Anti-complementary activity of triterpenoides from fruits of Zizyphus jujuba. Biol Pharm Bull, 27, 1883-1886 (2004) https://doi.org/10.1248/bpb.27.1883
  22. Lee YE, Park HJ, Park CB, Hwang SM. Anti-inflammatory activity of Scutellaria baicalensis root extract in lipopolysaccharide-induced RAW 264.7 cells. Korean J Food Sci Technol, 53, 115-120 (2021)
  23. Lim MJ, Gu YR, Hong JH. Extraction solvent-dependent antioxidant activities and cancer cell growth inhibitory effects of Scutellaria baicalensis extracts. Korean J Food Preserv, 26, 566-575 (2019) https://doi.org/10.11002/kjfp.2019.26.5.566
  24. Lowenstein CJ, Snyder SH. Nitric oxide, a novel biologic messenger. Cell, 70, 705-707 (1992) https://doi.org/10.1016/0092-8674(92)90301-R
  25. MaCartney-Francis N, Allen JB, Mizel DE, Albina JE, Xie QW, Nathan CF, Wahl SM. Suppression of arthritis by an inhibitor of nitric oxide synthase. J Exp Med, 178, 749-754 (1993) https://doi.org/10.1084/jem.178.2.749
  26. Ministry of Food and Drug Safety. Health functional food evaluation guide (related to stomach health). Available from: https://www.mfds.go.kr/brd/m_1060/view.do?seq=14659&srchFr=&srchTo=&srchWord=&srchTp=&itm_seq_1=0&itm_seq_2=0&multi_itm_seq=0&company_cd=&company_nm=&page= Accessed Oct. 23, 2019.
  27. Moncada S, Palmer RM, Higgs EA. Nitric oxide: Physiology, pathophysiology, and pharmacology. Pharmacol Rev, 43, 109-142 (1991)
  28. Nathan C, Xie QW. Nitric oxide synthase: Roles, tolls, and controls. Cell, 78, 915-918 (1994) https://doi.org/10.1016/0092-8674(94)90266-6
  29. Ogushi K, Lim P, Barr AR, Takahashi S, Asakura T, Ito K. Japanese barley meets australia: Quality performance of malting barley grown in different countries. J Inst Brew, 108, 303-309 (2002) https://doi.org/10.1002/j.2050-0416.2002.tb00555.x
  30. Park SG, Kim YK, Park JC, Lee CH, Kang CS, Kim KH, Park TI, Yoon YM. 'Heugho': A variety of black malting barley (Hordeum vulgare L.) with high antioxidant capacity and good brewing quality. Korean J Breed Sci, 54, 488-496 (2022) https://doi.org/10.9787/KJBS.2022.54.4.488
  31. Shin S, Shin Y, Lee J, Lee A, Kim M, Park C, Seo B, Roh SS. Ethanol-heated processed Scutellariae radix imporve inflammatory response through and inhibitory effect against oxidative stress in mice with the lipopolysaccharide-induced intestine injury of mice. Kor J Herbol, 30, 81-88 (2015) https://doi.org/10.6116/kjh.2015.30.4.81.
  32. Um JN, Min JW, Joo KS, Kang HC. Enhancement of antioxidanat and whitening effect of fermented extracts of Scutellariae baicalensis. J Soc Cosmet Sci Korea, 43, 201-210 (2017)
  33. Yoon SB, Han HS, Lee YJ. Effect of Scutellaria baicalensis extract on the proinflammatory mediators in Raw 264.7 cells induced by LPS. Kor J Herbology, 26, 75-81 (2011)
  34. Yun, HJ, Lee YJ, Kang MS, Baek JH. Inhibitory effect of Coicis semen extract (CSE) on proinflammatory mediatory. J Pediatr Kor Med, 23, 159-171 (2009)
  35. Zhang YB, Choi HJ, Han HS, Park JH, Son JH, Bae JH, Seung TS, An BJ, Kim HG, Choi C. Chemical structure of polyphenol isolated from Korean pear (Pyrus pyrifolia Nakai). Korean J Food Sci Technol, 35, 959-967 (2003)