DOI QR코드

DOI QR Code

Rhizoctonia solani AG 2-2IIIB에 의한 마 뿌리썩음병의 한국 내 발생

Occurrence of Stem Canker and Tuber Rot on Yam Caused by Rhizoctonia solani AG 2-2IIIB in Korea

  • 홍성기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이재국 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이영기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김완규 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 심홍식 (농촌진흥청 국립농업과학원 작물보호과)
  • Hong, Sung Kee (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Jae Kook (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Young Kee (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Sang Yeob (Agricultural Microbiology Division, NAAS, RDA) ;
  • Kim, Wan Gyu (Agricultural Microbiology Division, NAAS, RDA) ;
  • Shim, Hong Sik (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
  • 투고 : 2012.11.12
  • 심사 : 2012.12.03
  • 발행 : 2012.12.31

초록

2011년 안동과 진주의 마 재배포장에서 줄기 지제부 및 괴근썩음 증상이 나타났다. 병징을 나타내는 부위로부터 Rhizoctonia와 유사 속에 속하는 20개 균주가 분리되었다. rDNA-internal transcribed spacer(ITS) 염기서열 상동성을 기초로 8균주는 Rhizoctonia solani, 12균주는 Ceratobasidium sp.로 동정되었다. rDNA-ITS 염기서열의 cluster 분석에 의해 R. solani에 속하는 8개 균주 중 7개 균주는 균사융합군 AG 2-2IIIB, 1균주는 AG 1-1A에 속하였다. 또한, Ceratobasidium sp.에 속하는 12균주 중 7균주는 AG-Fa, 3균주는 AG-A, 나머지 2균주는 각각 AG-Fb와 AG-O에 속하였다. R. solani AG 2-2IIIB 균주들은 마의 줄기와 괴근에 병원성이었으나 R. solani AG 1-1A와 모든 Ceratobasidium sp. 균주는 비병원성이라는 것이 확인되었다. 이 결과는 조사지역에서 R. solani AG 2-2IIIB가 마의 줄기 및 괴근썩음병을 일으키는 중요한 병원균이라는 것을 나타낸다. 이 연구는 국내에서 R. solani AG 2-2IIIB에 의한 마 뿌리썩음병에 대하여 처음으로 보고하는 것이다.

Stem canker and tuber rot symptoms were observed on yam grown in Andong and Jinju, Korea in 2011. A total of 20 isolates of Rhizoctonia and allied fungi were obtained from the symptomatic plants. Among the isolates, 8 isolates were identified as Rhizoctonia solani and 12 isolates as Ceratobasidium sp. based on rDNA-internal transcribed spacer (ITS) sequence similarity. In the cluster analysis of rDNA-ITS sequences, 7 isolates of R. solani belonged to AG 2-2IIIB and remaining one to AG 1-1A. In addition, among the 12 isolates of Ceratobasidium sp., 7 isolates belonged to AG-Fa, three isolates to AG-A and the other two isolates to AG-Fb and AG-O, respectively. Pathogenicity tests showed that all the R. solani AG 2-2IIIB isolates are pathogenic on stem and tuber of yam but R. solani AG 1-1A and all the Ceratobasidium isolates are non-pathogenic. The results indicate that R. solani AG 2-2IIIB is an important pathogen causing stem canker and tuber rot on yams grown in the study areas. This is the first report of R. solani AG 2-2IIIB causing stem canker and tuber rot of yam in Korea.

키워드

참고문헌

  1. Ahn, J. H., Son, K. H., Sohn, H. Y. and Kwon, S. T. 2005. In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Kor. J. Plant Biotechnol. 32:317-223. https://doi.org/10.5010/JPB.2005.32.3.217
  2. Bai, Q., Wang, N. and Gao, J. 2010. First report of seedling blight caused by Rhizoctonia solani on Dioscorea nipponica in China. Plant Disease. 94:915.
  3. Blazier, S. R. and Conway, K. E. 2004. Characterization of Rhizoctonia solani isolates associated with patch diseases on turfgrass. Proc. Oklah. Acad. Sci. 84:41-51.
  4. Carling, D. E. 1996. Grouping in Rhizoctonia solani by hyphal anastomosis reaction. In: Sneh et al. (eds) Rhizoctonia species: Taxonomy, molecular biology, ecology, pathology and disease control. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 37-47.
  5. Kim, W. K., Hwang, Y. S. and Yu, S. H. 2008. Two Species of Penicillium associated with blue mold of yam in Korea. Mycobiology. 36:217-221. https://doi.org/10.4489/MYCO.2008.36.4.217
  6. Kim, W. G., Cho, W. D. and Ryu, H. Y. 1995. Diagnosis and control of Rhizoctonia diseases on crops. National Agricultural Science and Technology Institute, Suwon. (in Korean).
  7. Kim, W. G., Koo, H. M., Kim, K. H., Hyun, I. H., Hong, S. K., Cha, J. S., Lee, Y. K., Kim, K. H., Choi, H. S., Kim, D. G. and Park, B. Y. 2009. List of plant diseases in Korea. 5th ed. Korean Society of Plant Pathology, Anyang. (in Korean).
  8. Ozawa, S., Obata, H., Yasuoka, S. and Tanaka, F. 1994. Occurrence of root rot of Chinese yam [Dioscorea opposita], caused by Rhizoctonia solani Kuehn, in Hokkaido. Annu Rep. Soc. Plant Protect. N. Jpn. 45:43-44. (in Japanese).
  9. Salazar, O., Schneider, J. H. M., Julian, M. C., Keijer, J. and Rubio, V. 1999. Phylogenetic subgrouping of Rhizoctonia solani AG 2 isolates based on ribosomal ITS sequences. Mycologia. 91:459-467. https://doi.org/10.2307/3761346
  10. Sharon, M., Kuninaga, S., Hyakumachi, M. and Sneh, B. 2006. The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping. Mycoscience. 47:299-316. https://doi.org/10.1007/s10267-006-0320-x
  11. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. doi:10.1093/molbev/msr121.
  12. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22:4673-4680. https://doi.org/10.1093/nar/22.22.4673
  13. White, T. J., Bruns, T. D., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal genes from phylogenetics. 315-322. In: Innis MA., Gelfrand DH, Sninsky JJ and White TJ Eds. PCR Protocols. Academic Press, SanDiego, California, USA.