Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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The chemical constituents, antioxidant activity and anti-inflammatory activities of Solanum nigrum Linne by different harvest time

수확시기별 까마중 전초의 성분, 항산화활성 및 항염증 활성

  • Choi, Seongkyu (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Seo, Kyoung-Sun (Jangheung research institute for mushroom industry) ;
  • Kim, Yong-Doo (Department of Food Science and Technology, Sunchon National University) ;
  • Yun, Kyeong-Won (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Choi, Bong-suk (Jangheung research institute for mushroom industry) ;
  • Jin, Seong-Woo (Jangheung research institute for mushroom industry) ;
  • Kang, Kyeong-Yun (Department of Pharmacy, Sunchon National University) ;
  • Cho, In-Kyung (Jangheung research institute for mushroom industry) ;
  • Kim, Kyung-Je (Jangheung research institute for mushroom industry)
  • 최성규 (순천대학교 한약자원개발학과) ;
  • 서경순 ((재)장흥군버섯산업연구원) ;
  • 김용두 (순천대학교 식품공학과) ;
  • 윤경원 (순천대학교 한약자원개발학과) ;
  • 최봉석 ((재)장흥군버섯산업연구원) ;
  • 진성우 ((재)장흥군버섯산업연구원) ;
  • 강경윤 (순천대학교 약학과) ;
  • 조인경 (남부대학교 식품영양학과) ;
  • 김경제 ((재)장흥군버섯산업연구원)
  • Received : 2016.06.13
  • Accepted : 2016.10.05
  • Published : 2016.11.30
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Abstract

In this study, we investigated the variation in free sugars, amino acids, antioxidant activity, and anti-inflammatory activity of Solanum nigrum Linne based on harvest time. Major amino acids identified by HPLC analysis were proline, histidine, and serine. The highest content of total amino acids were found in S. nigrum aerial parts and roots harvested on July 10th and August 10th. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum, and the free sugar content varied significantly with harvest time. The fructose content of S. nigrum decreased with as harvest time increased. The total polyphenol content of S. nigrum was highest in those harvested on August 30th. The antioxidant activity of ethanol extract from S. nigrum collected at different harvest times were measured by DPPH and ABTS radical scavenging assays. The anti-inflammatory activity of these extracts were assayed via nitric oxide suppression in C6 glioma cells with a lipopolysaccharide (LPS)-induced inflammatory response. The anti-inflammatory activity and antioxidant effects were the highest in the extract from S. nigrum collected on August 30th. Good correlations were observed between antioxidant and anti-inflammatory activities in ethanol extract of S. nigrum roots harvested on August 30th.

본 연구는 까마중 수확시기에 따른 성분변화, 항산화효과 및 항염증 효과를 분석하여 최적 수확시기를 구명하고자 수행하였다. 까마중의 주요 아미노산은 수확시기에 관계없이 proline, histidine 과 serine으로 나타났으며, 구성아미노산 함량은 7월 10일과 8월 10일 수확한 까마중 지상부와 지하부에서 높게 나타났다. 4가지 유리당이 검출되었으며, 유리당 함량은 수확시기에 따라 편차를 나타내었다. 유리당 중 fructose는 수확시기가 늦어짐에 따라 감소하였으며, 총 폴리페놀 함량은 8월 30일 수확한 까마중 뿌리에서 가장 높게 나타났다. 수확기별 까마중 ethanol 추출물들의 항산화효과와 항염증효과 검정을 위하여 DPPH radical scavenging assay와 ABTS radical scavenging assay를 수행하였으며, 항염증 효과 측정을 위하여 lipopolysaccharide(LPS)로 염증을 유발시킨 C6 glioma cells을 NO 저해실험에 사용하였다. 항산화효과와 항염증 효과는 8월 30일 수확한 까마중 뿌리 ethanol 추출물에서 가장 높은 활성을 나타내었다. 8월 30일 수확한 까마중 뿌리는 총 폴리페놀 함량, 항산화 효과 및 항염증 효과간의 양적인 상관관계를 나타내었다.

Keywords

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