Purpose: This study was conducted in order to determine the effects of a health promotion program on perception of health behavior, physical fitness, and body composition in child care helpers. Methods: The study had a one group pretest-posttest quasi-experimental design. Data were collected from 38 child care helpers from July 6 to November 9, 2013. The health promotion program consisted of health education, self-exercise, encouragement, and identification. The following data were collected perception(perceived benefit, barrier, and self-efficacy), physical fitness(balance, grasp power, and flexibility), and body composition(body mass index, body fat ratio, waist circumference, and basal metabolic rate) and analysis was performed using on SPSS WIN 18.0. Results: Self-efficacy showed a significant increased. Body mass index, body fat ratio, and waist circumference showed a significant decreased. No significant difference in perceived benefit, barrier, physical fitness, and basal metabolic rate was observed between pre-test and post-test. Conclusion: This health promotion program had an effect on self-efficacy and body composition in child care helpers. Therefore, this health promotion program for child care helpers can be recommended as an effective nursing intervention.