An experiment was conducted to know ultra-pure distilled water penetration depth through large vessel, small vessel, latewood fiber and earlywood fiber in longitudinal direction of Castanea crenata. In heartwood, latewood fiber transported water more than large and small vessel. While in sapwood, small vessel conduction depth was found the highest. Penetration depth of water after 15.0 seconds, no significant difference was observed among earlywood fiber, latewood fiber and earlywood vessel. Whilst in heartwood, no statistical difference was observed among earlywood fiber, latewood fiber and earlywood vessel. At the beginning, the speed of water penetration was high and then gradually decreased.